摘要
用CO2激光(波长10600nm,辐射剂量20.1mW/mm2)对萌动小麦种子分别辐照0、1、3、5min,待其长至12d时,用10%(W/V)PEG6000胁迫其幼苗。结果表明:CO2激光处理1、3、5min显著提高了还原型谷胱甘肽(GSH)含量,显著降低了氧化型谷胱甘肽(GSSG)含量(5min除外),导致GSH/GSSG比率显著上升。3min激光处理显著提高了干旱胁迫下小麦幼苗叶片谷胱甘肽还原酶(GR)和谷胱甘肽-S-转移酶(GST)活性。通过参与降解因干旱胁迫而过量产生的过氧化产物,实现了细胞解毒功能。此外,1min和3min激光处理可显著提高干旱胁迫下小麦幼苗抗坏血酸氧化酶(APX)活性和抗坏血酸(AsA)含量,提高了组织内部的抗氧化能力,从而起到保护作用。
The objective of this study was to determine the effects of CO2 laser （wavelength 10600 nm, power density 20. 1 mW/mm^2 ） on glutathione dependent antioxidative system in wheat seedling under drought stress. Germinating seeds were exposed to CO2 laser radiation for 0 min, 1 min,3 min and 5 min, respectively,then they were treated with 10% （W/V） PEG6000 solution for 8 days. When the seedlings were 12-day-old, changes in the concentration of oxidized glutathione （GSSG）, reduced glutathione （ GSH ）, ascorbate （ AsA ） and the activities of glutathione reductase （ GR）, glutathione-S-transferase （ GST）, ascorbate peroxidase （APX） were measured to determine the effects of laser. The results showed that suitable laser treatment of seeds improved drought tolerance in wheat seedlings by decreasing the concentration of GSSG and increasing the activities of APX, GR, GST, AsA and GSH concentration. The results suggested that those changes in glutathione dependent antioxidative system were responsible for protection wheat against drought induced oxidative damage.
出处
《武汉植物学研究》
CSCD
北大核心
2008年第4期402-406,共5页
Journal of Wuhan Botanical Research
基金
国家自然科学基金资助项目(30670366)
