摘要
采用纤维素酶法从野木瓜中提取水溶性粗多糖,通过正交试验确定了最佳提取条件:提取温度50℃,pH 4.0,提取时间2.5 h,酶用量50U/g.粗多糖经蛋白酶与Sevage法相结合脱蛋白、大孔树脂脱色及水浴透析,得到野木瓜水溶性精多糖CCP,再经DEAE-纤维素阴离子交换层析柱,得一种主要洗脱组分CCP1.纸层析和Sepharose C l-6B色谱柱分析表明CCP1为多糖纯品,苯酚-硫酸比色法测得该多糖的糖含量为97.3%,紫外光谱分析未见蛋白质(280 nm)与核酸(260 nm)的特征吸收峰,红外光谱分析发现典型多糖吸收峰.结果表明,CCP1是初次从该植物中提取分离出来的新的均一多糖组分.
In this paper, first, crude water-soluble polysaccharides were extracted from Chaenomeles cathayensis using cellulase, and the optimal extraction conditions were determined via orthogonal experiments, that is, extracting the polysaccharides with a cellulose dosage of 50 U/g at 50 ℃ and pH 4.0 for 2.5 h. Next, the proteins in the crude polysaccharides were removed by means of Sevage method combined with acid-resistant proteinase. Then, the pigments in the polysaccharides were removed using macroporous resin and other small-molecule impurities were removed via dialysis. Thus, fine water-soluble Chaenomeles cathayensis polysaccharides （CCP） were obtained and further fractionated. Finally, an elution component CCP1 was separated via the column chromatography of DEAE- cellulose. According to the results of paper chromatography and Sepharose Cl-6B column chromatography, CCPI is as a kind of pure polysaccharide with a saccharide content of 97.3 % detected by the phenol-sulfuric acid assay. Moreover, as there are no absorption peaks of protein （ at 280 nm） and nucleic acid （ at 260 nm） in the UV spectrum but there are typical characteristic absorption peaks of polysaccharides in the IR spectrum, it is concluded that CCP1 is a new kind of homogeneous polysaccharide separated from Chaenomeles cathayensis.
出处
《华南理工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2008年第7期128-133,共6页
Journal of South China University of Technology(Natural Science Edition)
基金
贵州省科技基金资助项目(黔科合J字[2005]2017号)
贵州省自然科学基金资助项目(19993105)
关键词
野木瓜
多糖
提取
纯化
结构分析
Chaenomeles cathayensis
polysaccharide
extraction
purification
structural analysis
