摘要
蛇莓为民间常用草药,通常与其它中药配合使用,用于多种癌症的治疗。为了解其抗肿瘤活性成分,本文对其化学成分进行了研究。从该植物的酚性部分中分得两个酚性成分,分别鉴定为鞣花酸(Ellagic acid,EA)和短叶苏木酚羧酸(Brevifolincarboxylic acid,BA)。EA为首次从该属植物中分得。并首次建立了蛇莓中EA和BA的HPLC含量测定方法,样品以50%乙醇为溶剂超声提取30 min,提取液以C18色谱柱进行分离。以25 mM磷酸二氢钾缓冲液(pH=2.4)-乙腈进行梯度洗脱,流速1.0 mL/min,360 nm下检测。结果显示,EA和BA均具有良好的线性范围(r2>0.999)、精密度(RSD<0.50%)和重现性(RSD<1.50%),回收率分别为99.65%和102.95%。当进样体积为20μL时,EA和BA的最低检测限分别为12和20 ng/mL。以上结果表明该方法简便易行,结果准确,重复性好,可用于蛇莓中EA和BA的含量测定。
Duchesnea indica is a traditional Chinese medicine commonly used for treatment of cancers in combination with other herbs. Two phenolics ,ellagic acid （EA） and brevifolincarboxylic acid （BA） ,were isolated from the phenolicfraction of the herb. In addition, a new RP-HPLC method for simultaneous determination of EA and BA was developed and validated. The separation was performed on a C18 column, gradiently eluted with 25 mM potassium dihydrophosphate buffer （ pH 2.4） and acetonitrile at 1.0 mL/min and monitored at 360 nm. The plant materials were extracted with ethanol-water （50： 50）. The two components EA and BA showed good linearity （P 〉0.999） within the studied concentration ranges,with LOD of 12 and 20 ng/mL,respectively. The precision （RSD） was less than 1.50% for both two compounds and the extraction recoveries were 99.65% and 102.95% ,for EA and BA, respectively, The developed method was applied to assay the two components in the herbs of Duchesnea indica.
出处
《天然产物研究与开发》
CAS
CSCD
2008年第4期667-671,共5页
Natural Product Research and Development
基金
国家重点基础研究发展计划(973计划)(2004CB72030)
关键词
蛇莓
鞣花酸
短叶苏木酚羧酸
HPLC
Duchesnea indica
ellagic acid
brevifolincarboxylic acid
HPLC
