摘要
通过对毛茛科的唐松草(ThalictrumL.)及其近缘植物黄连、毛茛和芍药科的牡丹等植物的ITS序列和5S rRNA基因间隔区的克隆、扩增及构建系统发育树的分析研究,结果显示,唐松草扩增后ITS序列长607 bp,5SrRNA基因基因间隔区序列长323 bp;系统树证明唐松草属与黄连属和毛茛属的亲缘关系较近,结合形态学与化学等其它学科证据,本研究结论支持唐松草和黄连在进化上更接近,为含木兰花碱生物碱及毛茛甙植物的鉴别以及毛茛科相关植物的亲缘关系初步提供了分子依据.
The ITS sequences and the 5S rRNA spacer region of Ranunculaceae Thalictrum simpler and other kindred plants including Coptis Chinensis, Ranunculus japonicus and Paeonia suffruticosa were cloned and anolgied. The 5S rRNA spacer region and the ITS sequence of Thalictrum simplex were 323 bp and 607 bp respectively. It was showed that there was a relative close relationship between Thalictrum simplex and Coptis Chinensis Franch, Ranunculus japonicus Thunb. According to morphology and other disciplines such as chemical evidence the results support the relationship between Thalictrum simplex and Coptis Chinensis Franch is closer. A molecular method to distinguish the plants which contain thalictrine and the relationship of the Ranunculaceae plant was established.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第4期951-955,共5页
Journal of Sichuan University(Natural Science Edition)
基金
重庆市教委基础研究基金(KJ050510)
