摘要
目的:建立高效液相色谱法测定对乙酰氨基酚注射液的有关物质。方法:采用Alltima C18色谱柱,流动相为0.05 mol.L-1醋酸铵溶液-甲醇(85∶15),检测波长245 nm。结果:对乙酰氨基酚与其杂质能完全分离,已知杂质对氨基酚在2.40～191.84 ng线性关系良好(r=0.9999),平均回收率为99.8%,RSD=0.91%(n=6)。结论:所用方法简便、快速、准确,可用于对乙酰氨基酚注射液的质量控制。
Objective：To establish a method for the determination of rataled substances in paracetamol injection by HPLC.Metheds：Alltima C18 colomn was used （250 mm×4.6 mm,5 μm）,the mobile phase used was 0.05 mol·L^-1 ammonium acetate solutionmethanol （85：15）,the detection wavelength was 284 nm.Results：There was a good seperation for paracetamol and other related substances.These was a good linear relationship of p-aminophenol in the 2.40-191.84 ng （0.9999）,and the average recovery was 99.8%.The limit of detection was 5 μg·ml^-1.Conclusion ：The method is rapid, sensitive, accurate and effective for testing related substances in paracetamol injection and for its content determination.
出处
《现代医药卫生》
2008年第17期2556-2557,共2页
Journal of Modern Medicine & Health
