摘要
【目的】研究大孔树脂吸附法分离纯化射干中异黄酮类成分。【方法】采用紫外分光光度计法测定射干异黄酮的含量,分别考察了HPD-300、HPD-400、NKA-9、AB-8、DM-301及D101等6种吸附树脂对异黄酮类的动态吸附及洗脱性能。【结果】HPD-300用于分离纯化异黄酮类成分,吸附和洗脱性能良好,吸附过程中上样液浓度为1.0 g生药/ml树脂,pH值为7.0,流出液流速为1 BV/h,树脂的饱和吸附容量为65.82 mg/ml树脂;洗脱过程采用水和10%乙醇洗脱除杂,5 BV70%乙醇梯度洗脱,流速为1 BV/h,洗脱率在90%以上,所得固体样品中异黄酮类成分含量可达62%以上。【结论】HPD-300用于富集异黄酮类成分效果较好,是一种理想的分离纯化介质。
[Objective] To study the separation and purification of isoflavones from Belamcanda chinensis （L.） DC. by macroporous resins. [Methods] The dynamic adsorption and desorption characteristics of macroporous adsorption resins including HPD- 300, HPD- 400, NKA- 9, AB- 8, DM - 301 and D101 for isoflavones were investigated. At the base of the investigation, the best macroporous adsorption resin was chosen. [ Results] The adsorption and elution characteristies of HPD- 300 used to separate and purify isoflavones was very good. In the course of adsorption, the concentration of adsorption solution was 1.0 g dried medicinal herb per ml resins, with about of pH 7.0 of adsorption solution, and the flowing rate was 1BV/h In the course of elution, the resin column chromatography was eluted gradiently with 5BV 70% EtOH after removing impurities with water and 10% EtOH. The elution rates was more than 90%, and the contents of isoflavones was up to 62% in the dried fractions of 70% ethanolic elutions. [Conclusions] Macroporous adsorption resin HPD-300 is suitable for purify the total isoflavones from Belamcanda chinensis （L.） DC.
出处
《武警医学院学报》
CAS
2008年第10期837-840,共4页
Acta Academiae Medicinae CPAPF
关键词
射干
大孔吸附树脂
异黄酮类
分离纯化
Belamcanda chinensis
Macroporous adsoption resin
Isoflavones
Separation and purification
