摘要
目的分析2006-2007/06广州市大学城内不同校区的流感疫情的病原体。方法在疫区采集咽拭子标本,用MDCK细胞培养法分离病毒,用豚鼠红细胞凝集试验进行鉴别,对阳性标本进行病毒传代,然后用红细胞凝集抑制试验进行分型。提取不同校区病毒株的RNA,通过RT-PCR方法扩增流感病毒血凝素全长基因,测序并采用DNAs-tar软件对其核苷酸序列进行分析。结果2006年,35份咽拭子标本中有20份标本(57.1%)在HA试验中呈阳性反应,HI试验显示均为H1型流感病毒;2007年,48份咽拭子标本中有19份标本(39.6%)在HA试验中呈阳性反应,HI试验显示均为H3型流感病毒。2006年,两所校区的流感病毒株血凝素基因的同源性为96%;2007年,3所校区的流感病毒株血凝素基因的同源性为99%。结论2006年-2007年发生在不同校区的流感疫情都是各由同一病毒株所引起的。
Objective To analyze and compare the influenza epidemic status and pathogen that happened in June of year 2006 and 2007 in different campuses in Guangzhou University City. Method The influenza viruses were isolated by MDCK cells from throat swabs and examined by hemagglutination（HA） test. The types of influenza viruses were identified by hemagglutination inhibition （HI） test. Genome RNAS of virus strains from different campuses were extracted, and the whole length DNA of hemagglutinin gene were amplified by RTPCR, sequenced and the nucleotide sequences were analyzed by DNAstar software. Results 20 out of 35 throat swabs（57.1% ） were examined positive by HA test in 2006, and were identified as H1 type by HI test. 19 out of 48 throat swab（39.6% ） were examined positive by HA test in 2007, and were identified as H3 type by HI test. The homologic rate of influenza viruses in hemagglutinin gene from 2 different campuses was 96% in 2006, and that of influenza viruses in hemagglutinin gene from 3 different campuses was 99% in 2007. Conclusion Influenza is easy to transfer in crowded community such as Guangzhou University City. The outbreak of influenza in different campuses in 2006 and 2007 were caused by one influenza virus strain in either year. It indicated that the isolation of initial patient was very important in the prevention of influenza outbreak.
出处
《预防医学情报杂志》
CAS
2008年第10期801-804,共4页
Journal of Preventive Medicine Information
关键词
流感
病毒分离
红细胞凝集抑制实验
流感测序
Influenza
Virus isolation
Hemagglutination inhibition （HI） test
Influenza sequence
