摘要
目的建立砂仁及其混淆品的高效液相色谱(HPLC)指纹图谱鉴定方法。方法采用RP-HPLC(DAD)法,梯度洗脱,测定阳春砂、海南假砂仁及艳山姜的指纹图谱,并作相似度比较分析。结果砂仁及其混淆品的HPLC指纹图谱有明显差异。结论HPLC指纹图谱具有重现性好,特征性强,方法简便等特点,可用于砂仁与其混淆品药材的鉴别。
Objective To establish HPLC fingerprint for the identification of Amomum villosum and its adulterants. Methods Chromatographic fingerprint of Amomum villosum, Amomum chinense and Alpinia zerumbet was determined by RP - HPLC （DAD） and the gradient elution mode was applied in chromatographic separation, data were analyzed by Fingerprint Similarity Evalution Software to compare the similarity of samples. Results There was an apparent difference in fingerprint between Amomum villosum and its adulterants. Conclusion HPLC fingerprint method is repeatable and feasible and can be suitable for identifying Amomum villosum and its adulterants.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2008年第8期1967-1968,共2页
Lishizhen Medicine and Materia Medica Research
