摘要
目的研究红算盘子的化学成分及其细胞毒活性。方法采用多种色谱方法分离纯化，根据理化性质和各种波谱技术进行结构鉴定；用MTT法评价其细胞毒活性。结果从醋酸乙酯部位分离得到6个三萜类化合物，鉴定为算盘子二醇①，羽扇豆-20（29）-烯-3α，23-二醇②，算盘子酮③，表-羽扇豆醇④，3β，19α，23α-三羟基-12-烯-28-齐墩果酸⑤，2β，3β，23α-三羟基-12-烯-28-齐墩果酸⑥。结论以上化合物均为首次从红算盘子中分离得到；其中化合物1～4对人肝癌BEL-7402细胞，化合物5，6对非小细胞肺癌A-549细胞具有一定程度的细胞毒作用。
Objective To study the chemical constituents of Glochidion coccineum and their cytotoxicity in vitro. Methods Various column chromatographic technologies were applied for isolation and purification and the structures were elucidated by spectral evidence. MTT method was applied to investigate their cytotoxic activities. Results Six compounds were isolated from the part of ethyl acetate and identified as Glochidiol （1）, Lup - 20 （29） - erie - 1 α, 23 - diol （2）, Glochidone （3）, Epi - lupeol （4）, 3 β,19α, 23α- trihydroxy- 12- oleanen-28 -oic acid （5）, 2β,3β,23α- trihydroxy- 12 -oleanen- 28 -oic acid （6）. Conclusion The above compounds were obtained from this plant for the first time; compounds 1 -4 showed cytotoxic activities to human hepatoma （BEL- 7402）, as well as compounds 5 and 6 to lung adenocarcinoma （A -549 ）cell lines.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2008年第8期1931-1932,共2页
Lishizhen Medicine and Materia Medica Research
基金
贵州省中药现代化专项项目(No.黔科合农字20065041)
关键词
红算盘子
三萜
细胞毒
Glochidion coccineum
Triterpene
Cytotoxicity
