摘要
对PEG渗透胁迫下河北杨叶片的生理响应及其内在关系进行研究。结果表明:在PEG溶液的渗透胁迫下,河北杨叶片相对含水量逐渐下降,脂质过氧化产物丙二醛(MDA)积累,细胞膜相对透性破坏;在胁迫24h时,超氧化物歧化酶(SOD)和过氧化物酶(POD)活性显著下降,并伴随着O2.-产生速率的大幅度增加;但在胁迫18h时,SOD、POD活性及脯氨酸和可溶性糖含量的显著升高可以起到一定的减轻伤害的作用。相关分析结果表明:MDA含量及O2.-产生速率与相对含水量均呈极显著负相关;而SOD、POD活性与相对含水量相关性不显著;SOD活性与O2.-产生速率相关性显著,而POD活性与O2.-产生速率相关性不显著。上述指标间相关性说明河北杨对水分胁迫的响应是多种生理生化过程综合作用的结果,各生理指标间存在着一定的内在联系。
The physiological responses and their correlations of Populus hopeiensis leaves during PEG osmotic stress were investigated in this paper. The results showed that under the osmotic stress of PEG solution, the relative water content decreased, the content of malondialdehyde （MDA） increased and cell membrane selective permeability was deteriorated during the process of stress. After 24 h of stress,the O2^＋ production rate increased while the activities of superoxide dismutase （SOD） and peroxidase （POD） decreased. However, the activities of SOD and POD, the content of proline and soluble sugar increased at 18 h of treatment with the PEG solution, which might alleviate the damage of the stress. Correlation analyses showed that there was a significant negative correlation between the relative water content and MDA content or O2^＋ production rate respectively, whereas there was no significant correlation between the relative water content and the activities of SOD and POD. SOD activities were significantly and negatively correlated with O2^＋ production rate while no significant correlation was found between the relative water content and POD activities. The relationship between the above physiological indexes indicated that the responses of Populus hopeiensis leaves during PEG osmotic stress were the results of various physiological processes,and there existed some close correlations among the indexes in some way.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2008年第8期56-61,共6页
Scientia Silvae Sinicae
基金
国家林业局重点项目(2006-35)
甘肃省科技攻关项目(2GS064-A41-003-01)
关键词
河北杨
PEG渗透胁迫
生理响应
相关性分析
Populus hopeiensis
PEG
osmotic stress
physiological responses
correlation analysis
