摘要
为了研究固定化微生物在土壤生物修复中的应用,以实验室筛选出来的高效降解菌Q5为生物活性物质,利用生物大分子仿生合成出的纳米多孔氧化硅为载体,通过表面吸附固定化方法将其固定,制备出固定化微生物。考察固定化微生物初始pH值、温度、摇床转速和菌种的接种量对喹啉去除的影响,得到适宜的去除条件,在相同条件下比较固定化微生物与游离菌种对底物的去除情况,研究单一固定化菌种对不同浓度的喹啉的去除情况,考察固定化微生物的稳定性。实验结果表明,菌株Q5经固定化后,对喹啉的去除能力大大增强,在500 mg/L浓度下,40 h固定化Q5对底物去除率达96.6%,远高于未固定化Q5的去除率56.1%;对于高底物浓度,固定化微生物的去除效果明显,初始底物浓度为1 500 mg/L,反应70 h后去除率为91.6%,且这种固定化微生物的重复使用性能良好。
To study the application of immobilized microorganisms in bioremediation of soil, screened highefficiency degrading bacteria Q5 as bioactive substances and nanoporous silica synthesized by bio-macromolecule bionic technology as a carrier, immobilized microorganisms were obtained by surface adsorption. The optimum de- grading condition was found by evaluating the influences of the initial pH, temperature, shaking rate and inoculation amount. The degrading effect of immobilized microorganisms was compared with that of suspended bacteria under the same condition. The quinoline degradation by single immobilized bacteria with different quinoline concen- trations was studied and the stability of immobilized microorganisms was also observed. It was shown that the de- grading ability of strain Q5 was enhanced drastically after being immobilized. The degrading rate to quinoline of immobilized Q5 after 40 h reaction was 96.6% in 500 mg/L quinoline concentration, higher than that of the strain Q5 without immobilization （56.1%）. The degrading effect was also perfect （91.6% after 70 h reaction） in a high substrate concentration （1 500 mg/L） , and this kind of immobilized microorganisms was of good reusing property.
出处
《环境工程学报》
CAS
CSCD
北大核心
2008年第9期1265-1268,共4页
Chinese Journal of Environmental Engineering
基金
中国石油天然气集团公司中青年创新基金资助项目(06E1023)
关键词
固定化微生物
生物修复
喹啉
去除率
immobilized microorganisms
bioremediation
quinoline
remove rate
