摘要
目的采用两种技术检测糖尿病并发感染患者单核细胞趋化蛋白(MCP-1)mRNA与蛋白表达的变化。方法随机选择初诊糖尿病并发感染患者,应用酶联免疫吸附试验(ELISA)、竞争法逆转录聚合酶链反应(RT-PCR)及实时荧光定量PCR(FQ-PCR)检测患者感染治愈前、后MCP-1蛋白及mRNA定量水平;同时还分析了与其他参数的关系。结果糖尿病并发感染治疗前后趋化因子MCP-1 mRNA与蛋白表达均明显升高,MCP-1 mRNA(FQ-PCR)与蛋白表达水平呈正相关性,mRNA(竞争性密度比法)与其他结果未见明显相关性。结论利用FQ-PCR技术检测糖尿病并发感染患者MCP-1 mRNA与蛋白水平具有较高的一致性,对研究其病理机制和临床观察有重要意义。
Objective To detect the expression of monocyte chemoattractant protein-1（MCP-1） mRNA and protein in patients with diabetes mellitus （DM） complicated with infection with two RTPCR techniques. Methods 48 patients with preliminary diagnosed DM complicated with infection were enrolled in the study. Expression of MCP-1 mRNA and protein were detected with enzyme-linked immunosorbent assay （ELISA）, competitive polymerase chain reaction （RT-PCR）, and real time fluorescence quatitative PCR （FQ-PCR） before and after infection curing. The relationship between MCP-1 and other parameters was analyzed. Results The expression of mRNA and protein of MCP-1 were increased in the DM patients complicated with infection before and after treatment. There is a positive correlation in expression level between MCP-1 mRNA （measured by FQ-PCR） and protein; but there was no correlation between MCP-1 and other parameters. Conclusion High concordance was presented in MCP-1 mRNA and protein expression detected with FQ-PCR assay, which may play a key role in elucidation of pathomechanism and clinical observation during DM complicated with infection.
出处
《国际检验医学杂志》
CAS
2008年第8期700-702,共3页
International Journal of Laboratory Medicine
关键词
趋化因子CCL2
糖尿病
感染
临床实验室技术
Chemokine CCL2
Diabetes mellitus
Infection
Clinical laboratory techniques
