摘要
目的：研究松针油对人肝癌HepG2细胞的作用。方法：水蒸气蒸馏法提取松针油，GC-MS分析其成分，不同浓度松针油处理HepG2细胞后，用MTT法测定HepG2细胞的生长抑制率。HE染色及电镜下观察细胞凋亡形态的改变。流式细胞仪技术检测细胞凋亡率；流式细胞仪检查bcl-2基因表达水平；TRAP法测定端粒酶活性的变化。结果：MTT法证明松针油可显著抑制HepG2细胞生长，抑制率与药物浓度正相关，呈现明显的量-效和时-效关系。流式细胞仪可以检测到细胞凋亡，0．125～8mg／L的松针油能引起HepG2细胞凋亡，且呈时间依赖性及剂量依赖性。电镜下可观察到核浓缩及核碎裂等典型的细胞凋亡特征。松针油在诱导细胞凋亡的同时伴随端粒酶活性下调和bcl-2表达水平下降。结论：松针油诱导肝癌HepG2细胞凋亡，并抑制端粒酶活性，bcl-2参与了松针油诱导的肝癌HepG2细胞凋亡过程中端粒酶活性的调控。
Objective ： To study the effects of essential oil extracted from pine needles on HepG2 cell line. Methods ： HepG2 cells were treated with essential oil extracted from pine needles. Cell growth rate was determined with MTT assay, cell morphologic changes were examined under transmission electromicroscope and HE straining. Flow cytomett~ was used to exmine apoptotic cells. Bcl-2 gene expression was determined by flow cytometry and telomerase activity by TRAP assay. Results： Essential oils from pine needles could not only repress the growth of HepG2 cells significantly, but also induce apoptosis to them. Both dose-effect and time-effect relationship could be confirmed. Typical morphologychanges of apoptosis such as nuclear enrichment and karyorrhexis were observed through transmission eleetromicroscope and HE straining. Telomerase activity was down regulated in the essential oil extracted from pine needles induced apoptotic cells. The expression of bcl-2 gene was suppressed after the essential oil from pine needles treatement. Conclusion： The essential oil extracted from pine needles can inhibit cell growth of HepG2 cell line and induce apoptosis, which may associate with inhibition of telomerase activity and bcl-2 may be involved in the regulation of telomerase activity.
出处
《中药材》
CAS
CSCD
北大核心
2008年第8期1197-1200,共4页
Journal of Chinese Medicinal Materials
基金
广东药学院人才引进科研启动基金项目(2007JCX04)
关键词
松针油
HEPG2细胞
细胞凋亡
BCL-2基因
端粒酶
Essential oil extracted from pine needles
HepG2 cell line
Apoptosis
Bcl-2 gene
Telomerase
