摘要
应用RP-HPLC技术分析毛蚶中的抗癌活性肽。毛蚶活性肽的最优分离条件为:温度:30℃;波长:280nm;流速:1.0 ml/min;流动相A为80%乙腈(含0.1%TFA),流动相B为0.1%TFA水溶液,梯度洗脱。应用上述条件,成功地得到了10批样品的指纹图谱,并确定了14个共有峰。
RP-HPLC was applied to analyze active polypeptides in Area subcrenata, and the optimal condition for separation was also set up ： temperature ： 30℃ ; wavelength ： 280 nm; flow rate ： 1.0 ml/min ; Solvent A consisted of 80% acetonitrile and 0. 1% trif- luoroacetic acid（TFA） and solvent B contained 0. 1% TFA. In this condition, ten samples＇ fingerprints were gained, in one of which the genuine fraction exhibited fourteen ＂common peaks＂ representing the characteristics of the constituents.
出处
《中药材》
CAS
CSCD
北大核心
2008年第8期1134-1138,共5页
Journal of Chinese Medicinal Materials
基金
广东省科技计划重大专项(2004A30505003)
广州市科技计划重点项目(2005Z2-E4031)
关键词
毛蚶
反相高效液相色谱
指纹图谱
鉴定
Arca subcrenata Lischke
RP-HPLC
Fingerprint
Identification
