摘要
用放射免疫分析法,对9头持久黄体病牛中的7头子宫内灌注15甲PGF_2α2～8 mg/头(另2头不用药作对照)和8头卵巢静止病牛中的5头肌注LRH-I 400～600μg/头(另3头不用药作对照)的监测结果表明,持久黄体病牛血浆孕酮浓变为5.54±3.18 ng/ml,灌注15甲PGF_2α后1～3 d,血浆孕酮迅速下降,当降至<0.5 ng/ml时,母牛出现发情;卵巢静止病牛血浆17β-雌二醇浓度为55.91±18.29 pg/ml,肌注LRH-I后,17β-雌二醇浓度缓慢下降,1～3 d后回升,7～15 d达到峰值,母牛出现发情。
Nine dairy cows diagnosed of persistent corpus Luteum, were divid-ed into a experimental group (7) and a non treatment control (2). All in theexperimental group received intrauterine infusions of 15 methyl PGF_2α 2~8 mg.Eight animals diagnosed of inactive ovary were divided into a experimenal group(5), and a non treatment control (3). All in experimental group received an in-tramuscular: injection of LRH-Ⅱ400~600Ug.Before and after the treatments bloodsamples from each of these animals were collected. The study was to determinethe efficacy of two vehicles by employing radioimunoassay technic (RIA) tomeasure the circulating concentration changes of progesterone and oestradiol-17β.For animals suffering persistent corpus Luteum, the serum value of progesteronewas about 5.54±3.183 4 ng/ml ((?)±S), with highest peak value>10 ng/ml andlowest value>2.43 ng/ml. After 1~3 days' treatment, the serum progesteronelevel decreased very rapidly. When it reached 0.5 ng/ml, the cows showed sym-ptons of estrus. Before treatment the serum level of oestradiol-17β in the animalswith inactive ovary was about 55.91±18.29 pg/ml((?)±S), except for individualswith 80 pg/ml or more than that, the mean serum value ranged between 33~60pg/ml. After intramuscular injection of LRH-Ⅱ, the concentration of oestradiol-17β decreased gradually, then, 1~3 days later, the decrease reversed and 7~15days later, when oestradiol-17β reached its highest peak, the cows showed sym-ptons of estrus.
关键词
乳牛
动物疾病
前列腺素
激素
dairy cattle
animals diseases
hypothalamic releasing hormone
prostaglandin
