摘要
以植物双元表达载体pCAMBIA2300-35为基础,设计带有酶切位点KpnI和XbaI的一对引物,从克隆载体pGEM-NCED1扩增到目的基因TaNCED1,酶切回收后与同样双酶切的表达载体pCAMBIA2300-35连接,获得表达载体pTaNCED1,并将所构建的载体导入根癌农杆菌GV3101菌株,为该基因的功能鉴定及通过基因工程的方法提高小麦的抗逆性奠定了基础。
In this study, Triticum aestivum L.9-Cis-Epoxycarotenoid Dioxygenase gene（ TaNCED1 ） was amplified using specific primers containing restriction enzyme site of KpnI and XbaI. PCR products and the binary expression vector pCAMBIA2300 - 35S were digested by the corresponding restricted enzymes respectively, and linked directionally. The resuiting construction with TaNCED1 was named pTaNCED1. The pTaNCED1 was further introduced into Agrobacterium tumefaciens strain GV3101 for the following-up research such as gene functional identification and gene transformation for wheat stress tolerance improvement.
出处
《华北农学报》
CSCD
北大核心
2008年第4期77-80,共4页
Acta Agriculturae Boreali-Sinica
基金
山东省优秀中青年科学家基金科研奖励基金
