摘要
采用PCR-RFLP法对大白猪、杜洛克、长白猪、民猪和野家杂交猪的谷胱甘肽过氧化物酶5(Glutathion-Peroxi-dase 5,GPX5)基因、岩藻糖转移酶1(Fucosyltransferase1,FUT1)基因和核受体辅激活蛋白1(Nuclear receptor co-activator 1,NCOA1)基因进行多态性检测,结果表明:GPX5基因用HinfⅠ酶切可获得3种基因型,FUT1基因用HhaⅠ酶切可获得3种基因型,NCOA1基因用RsaⅠ酶切可获得3种基因型。同时对不同基因的基因型频率和基因频率进行计算,并分析相应基因的遗传效应。
For Glutathion-Peroxidase 5 （ GPX5 ）, Fucosyltransferasel （ FUT1 ） and Nuclear receptor co-activator 1 （ NCOA 1 ） gene, the target segment of those genes were amplified through PCR, and then these amplified segments in five strains of pig were genotyped by PCR- RFLP. The strains involved were Landrace Yorkshire, Duroc, Min pig and Crossbred pig. After genotyping by PCR-RFLP with Hinf Ⅰ in GPX5 gene obtained 3 genotypes ; With Hha Ⅰ in FUT1 gene obtained 3 genotypes;With Rsa Ⅰ in NCOA1 gene obtained 3 genotypes. Then the frequency of gene and genotype of these genes were calculated, and analysised for genetic effect.
出处
《华北农学报》
CSCD
北大核心
2008年第4期69-71,共3页
Acta Agriculturae Boreali-Sinica
