摘要
目的探讨线粒体DNA缺失对人乳腺癌细胞药物敏感性的影响。方法采用溴化乙锭(EB)处理获得人乳腺癌细胞MDA-MB-231的线粒体DNA缺失(rho0MDA-MB-231)细胞,电镜下观察细胞形态改变,胶原凝胶包埋三维立体培养法(CD-DST)对比分析细胞的药物敏感性,免疫组织化学方法检测P-糖蛋白(P-gp)、乳腺癌耐药蛋白(BCRP)的表达。结果与MDA-MB-231细胞比较,rhooMDA-MB-231细胞线粒体数量增多,嵴消失呈空泡样改变,对化疗药物相对抵抗,P-gp、BCRP的表达明显增加。结论线粒体的损伤可能参与了乳腺癌细胞耐药表型的形成。
Objective To explore the resistance in human breast cancer cells. Methods relationship between mitochondrial DNA deletion and drug The rho^0-derivative of MDA-MB-231 was generated by treating the cultured cells with ethidium bromide. The morphologies were confirmed by transmission electron microscope （TEM）. The sensitivities to chemotherapy were determined by Collagen Gel Droplet Embedded Culture-Drug Sensitivity Test （CD-DST）. P-glycoprotein（P-gp）and breast cancer resistance protein （BCRP） expression were detected by immunohistochemistry. All the differences were comparatively analyzed between MDA-MB-231 and rho^0 MDA-MB-231 cells. Results Compared with MDA-MB-231 cells, rho^0 MDA-MB-231 ceils showed different characters, including more mitochondria and cristae vacuolation, less drug sensitivity, and markedly higher expression of P-gp and BCRP. Conclusions Mitochondrial DNA deletion may play a role in the formation of drug resistance phenotype of human breast cancer.
出处
《中华乳腺病杂志（电子版）》
CAS
2008年第4期25-28,共4页
Chinese Journal of Breast Disease(Electronic Edition)
基金
国家自然科学基金(30470667)
关键词
线粒体
线粒体DNA
乳腺癌
多药耐药
Mitochondria
Mitochondrial DNA
Breast neoplasms
Multidrug resistance
