摘要
目的观察促红细胞生成素(EPO)在氧诱导视网膜病变鼠模型中的动态表达变化,初步研究EPO在早产儿视网膜病变(ROP)中的意义。方法将14只7d龄C57BL/6J幼鼠暴露于高氧环境5d后转置于正常氧环境5d,作为高氧组;另14只同日龄幼鼠为正常对照组。进行视网膜铺片和二磷酸腺苷(ADP)酶染色、组织病理学及免疫组织化学检测,分别观察视网膜血管的改变、视网膜新生血管细胞核数量及EPO蛋白的表达变化。结果相对低氧时,高氧组可见大量新生血管形成;EPO蛋白表达与视网膜新生血管的组织病理学改变相平行。结论EPO在不同时间点的动态表达变化与ROP的两期病理改变相一致,可能参与了ROP视网膜新生血管的形成过程。
Objective Retinopathy of prematurity（ROP）is a neovasculariztion and fiber tissue proliferative disease caused by undeveloped retinal vessels.The purpose of this study was to investigate the expression variance and signification of erythropoietin（EPO）in oxygen-induced retinopathy in mouse.Methods Fourteen one-week-old C57BL/6J mice were exposed to 75% oxygen for 5 days and to room air for another 5 days to establish the oxygen induced retinopathy model.Another fourteen mice were kept in room air as control group.The ADPase histochemical technique was applicated for retinal flatmount to assess the oxygen-induced changes of retinal blood vessels.The proliferative neovascular response was quantified by counting the endothelial cell nuclei of new vessels breaking through retinal inner membrance in paraffin section.Immunocytochemistry was performed on some of cross-section of the retina to detect EPO protein expression,and integrated optical density（IOD）was determined with image analysis system.Results Constriction and occlusion of the retinal blood vessels and decrease in central perfusion were found under the hyperoxia condition,and dilation and proliferation of blood vessels were found under the relatively hypoxia condition.The mean neovascular nucleus per cross-section was 20.2±0.78 in oxygen exposure group and 0.30±0.48 in the control group,showing a significant difference between them（t=-68.03,P〈0.001）.The EPO IOD in oxygen exposure group and control group was 45.61±0.59 and 24.91±0.64,showing a higher level in oxygen exposure group（t=30.89,P〈0.05）.The results of immunocytochemistry showed parallel corresponding relation between expression of EPO and neovascularization.Conclusion The expression changes of EPO protein at different time is coincident with the pathology of ROP,implying this cytokine may participate in retinal new vessel formation of ROP.
出处
《眼科研究》
CSCD
北大核心
2008年第8期590-593,共4页
Chinese Ophthalmic Research
