摘要
目的探讨急性坏死性胰腺炎(ANP)大鼠模型血液TNF-α、IL-10和糖皮质激素受体(GR)的变化规律及地塞米松(DEX)干预对上述指标的影响。方法48只雄性SD大鼠随机分为ANP组(A组,n=16)、DEX治疗组(D组,n=16)和假手术组(S组,n=16)。除S组大鼠外,各组均通过逆行胰胆管注射5%牛磺胆酸钠制作ANP模型;D组大鼠术后肌注地塞米松干预。制模后8、12 h分别处死各组一半大鼠,分别观察大鼠胰腺病理改变、血清淀粉酶、TNF-α、IL-10水平和全血有核细胞GR的mRNA表达等指标。结果①A组大鼠表现为典型ANP病理改变,包括间质水肿、炎细胞浸润、出血和胰腺坏死;D组大鼠胰腺的炎症反应相对较轻。②A组血清TNF-α、IL-10显著高于S组(P<0.01),D组血清TNP低于A组而IL-10高于A组(P<0.01)。③在术后8 h和12 h,D组血GR mRNA表达显著低于S组(分别P<0.05,P<0.01)。结论TNF-α、IL-10以及GR的改变与ANP的炎症反应存在相关性,外源性DEX可能通过影响这些因素达到缓解ANP的作用。
Objective To investigate the changes of TNF-α, IL-10, glucocorticoid receptor （GR） in Rats with acute necrotizing pancreatitis （ANP） and the influence of Dexamethasone（DEX） on these parameters in ANP rats. Methods Fourty-eight male Sprague-Dawley （SD） rats were randomly divided into acute-necrotizing-pancreatitis group （group A, n= 16）, DEX-treated group （group D, n= 16） and sham-operation group（group S, n= 16）. ANP model was induced by intergraded injection of 5% sodium tanrocholate to biliopancreatic duct in the rats except for group S, and the rats in group D were intramuscularly given dexamethasone after operation. The change of pancreatic pathology, the serum AMY, TNF-α and IL- 10 level, and the blood mRNA expression of GR were examined. Results （1） Typical ANP pathological changes were found in group A, including i.e. interstitial edema, infiltration of inflammatory cells, hemorrhage and parenchyma necrosis; and the mild inflammation was found in rats of D group which treated by Dexamethasone. （2） The concentration of serum TNF-α, IL-10 in group A were obviously higher than that in group S （P〈0.01）. The concentration of serum TNF-α decreasing and IL-10 increasing in rats were induced by the usage of Dexamethasone （P〈0.01）. （3） The mRNA expression of blood GR in group D was lower significantly at 8h or 12h after the operation than that in group S （P〈0.01 or P〈0.05）. Conclusions The changes ofTNF-α, IL-10, GR are involved in the inflammation response of rat ANP, and the dexamethasone can relieve ANP inflammation response by affecting these factors.
出处
《实验动物与比较医学》
CAS
2008年第4期230-233,共4页
Laboratory Animal and Comparative Medicine
