摘要
目的研究Smad2和Smad3蛋白及其活化形式P-Smad2、P-Smad3在人正常肾脏组织中的表达、定位及其意义。方法采用免疫组织化学技术(SP法)检测20例人肾脏中Smad2、Smad3蛋白及P-Smad2、P-Smad3的表达和定位。结果Smad2、Smad3在肾小管、肾小球和集合小管中广泛表达,主要定位于细胞质,其中远端小管曲部呈强阳性;P-Smad2、p-Smad3也在肾脏皮、髓质中广泛分布,主要定位于细胞核,远端小管更多见。结论Smad2、Smad3在正常肾脏中有着活跃的功能。
Objective To investigate the expression and localization of Smad2/3 and phosphorylated-Smad2/3 in human kidney. Methods Immunohistochemistry was used to analyze 20 specimens of human renal tissue. Results Smad2 and Smad3 proteins were widely expressed in renal tubules and corpuscles, and mainly localized within the cytoplasm of the epithelia lining the renal tubules and collecting ducts, especially remarkable in the epithelia of distal convoluted tubule. P-Smad2 and p-Smad3 were mainly distributed in the nuclei in renal cortex and modulla, with more frequent localization in distal convoluted tubules. Conclusion Smad2 and Smad3 play active roles in normal human renal function.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2008年第4期345-348,共4页
Chinese Journal of Histochemistry and Cytochemistry
基金
北京大学医学部985基金(61220600062)
