摘要
背景:运动训练对骨骼肌超微结构的影响是运动医学中定量化的细胞生物学研究热点。目的:观察大鼠耐力训练后减量训练模型骨骼肌肌浆网的钙离子转运功能的变化。设计、时间及地点:随机对照动物实验,动物训练于2006-04/07在西安体育学院进行,生化实验于2006-07在西安交通大学生物实验中心完成。材料:2月龄雄性健康SD大鼠39只,体质量(220±20)g。方法:5只SD大鼠用于预实验。其余大鼠随机分为正常对照组8只、耐力训练组8只、耐力减训组18只。耐力训练组和耐力减训组递增训练周期均为6周,6周后将耐力减训组根据减训周期随机分为耐力减训2周,4周,6周组。对训练大鼠采用小动物跑台进行递增耐力负荷训练,减训组采用逐渐减少运动强度训练。主要观察指标:使用酶偶联法测定大鼠腓肠肌肌浆网Ca2+-ATPase的活性;采用荧光分光光度计测量肌浆网最大Ca2+摄取量和释放量。结果:①肌浆网SRCa2+-ATPase活性:耐力训练组较正常对照组显著性增加(P<0.01);耐力减训2周组与耐力训练组比较基本不变(P>0.05);耐力减训4周,6周组较耐力训练组下降(P<0.01)。②肌浆网SR最大Ca2+摄取和释放率:耐力训练组显著性增加(P<0.01);耐力减训2周组与耐力训练组比较差异无显著性意义(P>0.05);耐力减训4周,6周组下降显著(P<0.01)。结论:耐力训练6周后,SD大鼠骨骼肌肌浆网Ca2+ATPase的活性增强,钙离子的摄取和释放率增加。减量训练2周骨骼肌肌浆网Ca2+ATPase的活性、钙离子摄取和释放率下降不明显。4周更长时间骨骼肌肌浆网Ca2+ATPase的活性及钙离子摄取和释放率下降明显。
BACKGROUND： Effect of exercise training on ultrastructure of skeletal muscle is a focus of quantified cytobiology in sports medicine. OBJECTIVE： To explore the changes of transportation function of calcium ion in sarcoplasmic reticulum of muscle in the model of reduced training after endurance training. DESIGN, TIME AND SETTING： The randomized animal trial was performed at Xi＇an Sports Institute from April to July 2006, and the biochemical test was performed at the Animal Experimental Center of Xi＇an Jiaotong University in July 2006. MATERIALS： Thirty-nine healthy 2-month-old male SD weighing （220±20） g were selected. METHODS： Five rats were used in the preliminary experiment, and the others were randomly divided to normal control group （n=8）, endurance training group （n=8） and reduced training group （n=18）, The increasing training period of endurance training group and reduced training group lasted for 6 weeks, and then reduced training group was randomly divided to 3 groups according to reduced training cycle： 2, 4 and 6 weeks group. The rats did increasing endurance loading exercise on a treadmill, while reduced intensity was used in reduced training group, MAIN OUTCOME MEASURES： Enzyme coupling method and fluorescence probe technology were used to detect sarcoplasmic reticulum Ca^2＋ -ATPase （adenosine triphosphatase） and maximum Ca^2＋ uptake and release of gastrocnemius muscle. RESULTS： The activity of Ca^2＋ -ATPase in endurance training group was significantly increased compared with normal control group （P 〈 0.01）; while reduced training 2 weeks group was similar to endurance training group （P 〉 0.05）; reduced training 4 and 6 weeks groups were significantly reduced compared with endurance training group （P 〈 0.01）. The maximum uptake and release of Ca^2＋ in sarcoplasmic reticulum of muscle of endurance training group was significantly increased （P 〈 0.01）, and there were no significant differences between reduced training 2 weeks group and endurance training group （P 〉 0.05）; while the maximum uptake and release of Ca^2＋ in sarcoplasrnic reticulurn of reduced training 4 and 6 weeks group was significantly reduced （P 〈 0.01）. CONCLUSION： Six-week endurance training enhances activity of Ca^2＋ -ATPase in sarcoplasmic reticulum of skeletal muscle and increases Ca^2＋ uptake and release; after reduced training for 2 weeks, activity of Ca^2＋ -ATPase, and Ca^2＋ uptake and release are slightly reduced, while significantly reduced after more than 4 weeks of reduced training,
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第33期6474-6478,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
西安工业大学校长基金资助(XAGDXJJ0640)~~
