摘要
目的探讨α-内收蛋白基因变异(Gly460Trp)及内皮型一氧化氮合酶基因(G894T、T786C)变异与大动脉弹性相关性研究。方法(1)采用Complior SP脉搏波速度测定仪测量股-颈动脉PWV(C-FPWV),共319例,其中C-FPWV异常组204例,对照组115例;(2)采用聚合酶链反应和限制性酶切的片段长度多态分析方法检测α-内收蛋白基因变异(Glv460Trp)及一氧化氮合酶(eNOS)基因变异(G894T、T786C)。结果(1)C-FPWV升高组收缩压(SBP)、舒张压(DBP)均显著高于对照组(P<0.05);(2)C-FPWV异常组WW、TG+TT基因型、W、T等位基因频率显著高于对照组(P<0.05)。C-FPWV异常组T786C(CT、CC)基因型及C等位基因频率与对照组比较差异无显著性(P>0.05);(3)WW和TG+TT基因型与WG+GG、TT基因型比较C-FPWV、收缩压、舒张压差异有显著性(P<0.05);(4)Logistic回归结果显示:α-内收蛋白460WW基因型(P=0.001,OR=3.234,95%CI 1.655～6.320)及内皮型一氧化氮合酶(eNOS)基因G894T多态性TF、TG基因型是动脉弹性减退的危险因素(P=0.025,OR=0.477,95%CI 0.249～0.911)结论α-内收蛋白基因变异(Glv460Trp)及一氧化氮合酶基因变异(G894T)与大动脉弹性有密切相关性,WW、TF、TG型是大动脉弹性减退的敏感基因型。
Objective To assess the association beween -Adducin and Endothelial Nitric Oxide Synthase Gene polymorphism and big artery elasticity. Methods （ 1 ） Big artery elasticity was measured with Complior SP automatic pulse wave measurement system of pulse wave velocity（PWV） in 319 subjects ,including 204 subjects with an abnormal C-FPWV and 115 normal controls; （2）The genetypes were determined with polymerase chain reaction and allele-specific reaction enzyme analysis. Results （ 1 ） SBP and DBP in abnormal CFPWV were significantly higher than in normal. CFPWV （ P 〈 0.05 ） ; （2） The frequencies of W and T allele and TT ＋ TG genotypes in abnormal C-FPWV group were significantly higher than in control group （ P 〈 0.05 ）. The frequencies of C allele and T786C （ CT, CC） genotypes were not significantly different in two groups （P 〉 0.05 ） ;（ 3 ）C-FPWV ,SBP and DBP in WW and TT ＋ TG genotype were significantly different from that in WG ＋ GG and GG genotype （ P 〈 0.05 ） ; （ 4 ） Logistic regression analysis revealed that WW and TT ＋ TG genotypes were associated with abnormal C-FPWV （ P = 0. 001, OR = 3. 234,95% CI 1.655-6.320;P=0.025,0R =0.477,95%CI 0. 249-10.911）. Conclusion The α-Adducin （Gly460Trp） and eNOS（G894T） gene polymorphism are associated with big artery elasticity. WW,TT ＋ TG genotype is a sensitive genotype reflecting the abnormality of big arterial compliance.
出处
《中国分子心脏病学杂志》
CAS
2008年第4期208-213,共6页
Molecular Cardiology of China
基金
内蒙古自然基金(200508010916)
