摘要
α-亚麻酸(ALA)被称为必需脂肪酸,对人体有一系列的保健作用。ω-3脂肪酸脱氢酶(FAD)催化亚油酸(LA)生成ALA。大豆种子中ALA含量较高,为了比较高、低油大豆ω-3FAD的序列差异,用RT-PCR方法从大豆品种鲁豆11和郑9525的未成熟种子中扩增GmFAD3C的cDNA,获得的cDNA长度为1174bp,编码380个氨基酸。在推测的氨基酸序列中发现19处差异,为研究GmFAD3C基因在高、低油大豆种质中的差异表达奠定了基础。
α-linolenic acid（ALA） is an essential fatty acid which has a lot of healthcare functions to human. In plants, ω-3 fatty acid desaturases（FAD） catalyze linoleic acid（LA） to form ALA. The seed oil of soybean contains high level of ALA. RT-PCR was used to amplify the cDNA of GmFAD3C from the cultivars of Ludoul 1 and Zheng 9525. The length of the acquired cDNA,containing 380 amino acids,was 1174bp. Nineteen different amino acids were detected in the amino acid sequences of GmFAD3C from Ludoull and Zheng 9525. These differences will help us to research the differential expression of GmFAD3C between high-oil and low-oil soybean.
出处
《大豆科学》
CAS
CSCD
北大核心
2008年第4期569-571,共3页
Soybean Science
基金
山东省自然科学基金资助项目(Y2006D20)
山东省农业科学院青年科研基金资助项目(2005YQ007)
关键词
大豆
亚麻酸
ω-3脂肪酸脱氢酶
序列差异
Soybean
Linolenic acid
ω- 3 fatty acid desaturase
Sequence difference
