摘要
目的：建立复方蛋黄乳膏中VitB2的含量测定方法。方法：采用一阶导数分光光度法测定复方蛋黄乳膏中的VitB2含量,VitB2的检测波长范围为386-554 nm。结果：浓度范围为0.011 9-0.023 8 mg/ml,测定振幅D与浓度呈良好的线性关系（γ=0.999 2）,回收率为99.4%,RSD为0.4%。结论：本法可消除其它组分的干扰,简便、易行、准确,适合医院制剂的质量控制。
Objective： To establish a method to determine content of VitB2 in the compound yolk emulsion by the first order derivative spectrophotometry. Methods： The first order derivative spectrophotometry was used to determine content of VitB2 in the compound yolk emulsion. Results： The linear correlation coeffi- cient of the amplitude （D） at 386-554 nm with concentration of VitB2 in the compound yolk emulsion was 0. 999 2 in the range of 0. 011 9-0. 023 8 mg/ml,the average recovery of VitB2 was 99.41% （n =6）, the relative standard deviation （RSD） of which was 0. 42%Conclusion： The interferences of the other components can be excluded by this method; which is simple, rapid and accurate. It is suitable for the quality control of hospital preparation.
出处
《新疆医科大学学报》
CAS
2008年第6期660-661,共2页
Journal of Xinjiang Medical University
关键词
一阶导数分光光度法
复方蛋黄乳膏
VITB2
the first order derivative spectrophotometry
the compound yolk emulsion
VitB2
