摘要
实验采用饱和硫酸铵法从健康肉鸡胆囊收集的胆汁中粗提二聚体SIgA,继而透析粗提产物除盐,然后进行纤维素DEAE-52离子交换层析,使用紫外分光光度计测定收集液的蛋白浓度,对层析产物浓缩使其浓度≥2.0 mg/m l,最后使用SDS-PAGE进行鉴定,从电泳所得图像中可以看到清晰的两个条带,一个条带分子量在67～70 ku之间为重链,另一个条带在26～28 ku之间为轻链。进一步将纯化的鸡SIgA抗体免疫健康兔,制备得到兔抗鸡SIgA抗体。
The experiment intends to abstract and identify the chicken secretory immunoglobulin A, and Preparation of Rabbit anti - chicken SIgA. Dimeride SIgA is originally abstracted from the chicken bile by the method of saturated ammonium sulfate, desalination by dialysis, cellulose DEAE - 52 ion exchange chromatography, density the concentration of collected liquid by ultraviolet spectrophotometer, concentrate the laminar analysis production and make density greater than or equal to 2. 0 mg/mL, identify the laminar analysis production by SDS - PAGE. We can find two limpid bands from the electrophoresis figure, one is the H - chain, which molecular weight is between 67KDa and 70KDa, the other is the L - chain, which molecular weight is between 26KDa and 28KDa. then, the healthy rabbit was immuned by SIgA, and gain the rabbit anti - chicken SIgA.
出处
《贵州畜牧兽医》
2008年第3期5-7,共3页
Guizhou Journal of Animal Husbandry & Veterinary Medicine
