摘要
硫丹是一种有机氯杀虫剂,广泛应用于谷物、蔬菜、水果、茶叶等害虫的防治。然而,在杀灭害虫、提高农业产量的同时,硫丹对水生生物的生存构成威胁。为了丰富水生生物毒理学资料,评价硫丹对水生生物健康生长的风险,本文采用传统毒理学方法,在室内静态环境条件下,研究了硫丹对斑马鱼（Danio rerio）的毒性效应。结果显示24 h、48 h、72 h、96 h半数致死浓度（LC50）分别为4.24μg/L、2.49μg/L、1.77μg/L、1.62μg/L。在亚慢性实验条件下,硫丹对肝和脑组织中的超氧化物歧化酶及乙酰胆碱酯酶有显著影响。硫丹对斑马鱼超氧化物歧化酶活性具有促进效应,在0.17~0.74μg/L范围内对乙酰胆碱酯酶具有抑制效应。
Endosulfan is a kind of manufactured organochlorine pesticide. It is widely used to control insects in agriculture,including food crops such as grains, vegetables, fruits, and tea. Although endosulfan plays an important role in controlling insects and increasing crop production, it affects aquatic organism survival. The primary objective of the research provided information of aquatic toxicology and evaluated the survival risks of aquatic organism during acute and sub-chronic endosulfan exposure. The paper summarized the toxicological effects of endosulfan on Zebrafish （ Danio rerio ） in glass aquaria under static conditions. The results showed that the concentrations of endosulfan that killed 50% of the Zebrafish within 24 h,48 h,72 h,96 h LC50 were 4.24 μg/L,2.49 μg/L, 1.77 μg/L, 1.62 μg/L respectively. After being exposed to sub-lethal concentrations of endosulfan for 30 d, significant effects of endosulfan on superoxide dismutase （SOD） and acetylcholinesterases （ACHE） activity of Zebrafish had been found. The activity of SOD in livers and brains for experimental groups was significantly increased comparing with control groups. The activity of AChE in livers and brains was decreased in dose of endosulfan 0.17 μg/L and 0.74 μg/L. Key words：Endosulfan; Zebrafish; Suporoxide dismutase; Acetylcholinesterases; Activity
出处
《动物学杂志》
CAS
CSCD
北大核心
2008年第4期1-6,共6页
Chinese Journal of Zoology
基金
国家重点基础研究发展计划项目(No.2006CB403306)
中国科学院研究生科学与社会实践项目资助
关键词
硫丹
斑马鱼
超氧化物歧化酶
乙酰胆碱酯酶
活性
Endosulfan
Zebrafish
Superoxide dismutase
Acetylcholinesterases
Activity
