摘要
以云南特有濒危树种黑黄檀（Dalbergia fusca）的种子为材料，研究了脱落酸（ABA）对种子萌发的抑制作用，以及种子萌发过程中吲哚乙酸（IAA）、赤霉酸（GA3）、6-苄基腺嘌呤（6-BA）和乙烯利对ABA的拮抗作用。黑黄檀种子萌发的适宜温度为30℃。交替光照（14h光照和10h黑暗）以及黑暗对种子萌发没有明显的影响。0．001～0．1mmol／LABA不影响种子的萌发率，但降低种子的萌发进程；1mmol／L和2．5mmol／LABA显著地抑制种子的萌发率和萌发进程。种子的萌发率不被0．0001～1mmol／IAA和GA3、0．0001～0．1mmol／L6-BA、以及0．001～10mmol／L乙烯利（乙烯供体）的影响。但被1mmol／L6-BA抑制。1mmol／LABA对种子萌发的抑制作用能被0．01-1mmol／L IAA、0．01～1mmol／LGA3、0．001～0．1mmol／L6-BA和0．1～10mmol／L乙烯利所拮抗，而且这种拮抗作用与植物激素的类型和浓度有关。0．0lmmol／L6-BA和0．1mmol／L乙烯利对1mmol／LABA抑制作用的拮抗不能被添加0．001mmol／L IAA或者0．001mmol／L GA3加成。但0．1mmol／L乙烯利对1mmol／L ABA抑制作用的拮抗能够被添加0．01mmol／L6-BA或者0．1mmol／L6-BA加成，导致更高的萌发率和幼苗生长。
Seeds of Dalbergiafusca, an endangered tree species endemic to Yunnan province of China were used to study the inhibitory effects of abscisic acid （ABA） and the antagonism of IAA, GA3, 6-BA and ethephon to ABA in the seed germination. The optimum temperature for the seed germination was about 30℃. There was no different effects of ahernating photoperiod （ 14 h light and 10 h dark） and darkness on the seed germination. After treated by 0.01-0.1 mol/L ABA, the seed germination percentage was not affected, but the time course of germination was decreased, while those levels were dramatically inhibited by 1 mmol/L and 2.5 mmol/L ABA. The seed germination percentage was not affected by 0.0001- 1 mmol/L indole-3-acetic acid （IAA）, gibberellic acid （GA3）, 0.0001-0.1 mmol/L 6-benzyladenine （6-BA）, and 0.001 - 10 mmol/L ethephon （the ethylene donor）, but was inhibited by 1 mmol/L 6-BA. The inhibition effect of 1 mmol/L ABA on seed germination was antagonized by 0.01-1 mmol/L IAA, 0.01-1 mmol/L GA3, 0. 001- 0.1 mmol/L 6-BA, and 0.1-10 mmol/L ethephon, which were phytohormone type- and concentration-dependent. The antagonistic actions of 0.01 mmol/L 6-BA and 0.1 mmol/L ethephon to 1 mmol/L ABA inhibition could not be increased by addition of 0.001 mmol/L IAA or 0. 001 mmol/L GA3. The antagonistic action of 0.1 mmol/L ethephon to 1 mmol/L ABA inhibition, however, could be increased by addition of 0.01 mmol/L 6-BA or 0.1 mmol/L 6-BA, which resulting in higher germination percentage and enhancing seedling growth.
出处
《云南植物研究》
CSCD
北大核心
2008年第4期440-446,共7页
Acta Botanica Yunnanica
基金
Knowledge Innovation Program of the Chinese Academy of Sciences(KSCX2-SW-117,KSCX2-YW-Z-058)
关键词
脱落酸
黑黄檀
乙烯利
植物激素的相互作用
种子萌发
Abscisic acid
Dalbergia fusca
Ethephon
Phytohormone interaction
Seed germination
