摘要
为了建立花粉管通道介导的小麦转基因植株的抗生素筛选体系，将小麦种子播种于不同浓度的抗生素G418溶液中，7d后统计各处理小麦幼苗的苗重、苗高、根数、根长、胚芽鞘长等形态指标，结果表明，低浓度的G418即可显著抑制小麦幼苗的苗重、苗高和根长，而对胚芽鞘没有显著的抑制作用；小麦转基因植株G418适宜筛选浓度为25～50mg／L，根长是小麦转基因植株筛选的适宜的形态指标。
In order to establish the antibiotic selective system of transgenic plants of wheat via pollen tube pathway,the seeds of wheat were sowed in the solution contained different concentration of antibiotics G418. ANOVA analysis based on the morphological indexes of 7--day seedlings showed that low concentration of G418 significantly restrained the weight of seedlings, height of seedlings and length of roots,but not to the length of coleoptiles. The optical G418 concentration for the selection of transgenic wheat was about 25 to 50mg/L,and the root length was a proper morphological index to judge the transgenic wheat.
出处
《河南农业科学》
CSCD
北大核心
2008年第8期41-43,共3页
Journal of Henan Agricultural Sciences
基金
河南科技大学人才基金(05-006)
关键词
小麦
抗生素G418
敏感浓度
Wheat
Antibiotics G418
Susceptive concentration
