摘要
目的:研究紫杉醇(PA)联合白藜芦醇(RES)对人胃癌MGC803细胞株的影响。方法:采用MTT法检测两种药物单用和联合应用对MGC 803细胞的作用,光镜观察细胞结构变化,流式细胞术检测细胞周期分布。结果:PA可抑制MGC803细胞生长,呈剂量及时间依赖性(P<0.05);PA促进人胚肺成纤维细胞(HPF)生长,呈剂量依赖性(P<0.05);PA与RES联合应用对MGC803生长抑制有协同作用(P<0.05),光镜下细胞质内产生异常物质;PA对G0/G1期有阻滞作用,RES对S期有明显阻滞作用。结论:PA对胃癌MGC803细胞有抑制作用。PA与RES对胃癌MGC 803细胞抑制具有协同作用,与单用RES相比,PA与RES联合应用可减少RES的用药剂量。
Objective： To evaluate effect of combination of PA and RES on human gastric carcinoma cell MGC803 in vitro. Methods ：The effects of PA and RES were measured by MTT assay. Morphous of cell was observed by light microscope, Flow cytometry was used for MGC803 cell cycle analysis.Results： PA significantly inhibited the growth of MGC803 cell in a dose and time dependent way （P〈0.05）. Many abnormal material in cytoplasm was found under light microscope. PA significantly stimulated the growth of HPF dose dependently （P〈0.05）.A strong synergism was found between PA and RES in MGC803 cells. PA induced Go/Gl-phase arrest, and RES significantly induced S-phase arrest （P〈0.05） in MGC803 cells, Conclusions： PA can inhibit cell growth in gastric carcinoma cells but not in normal cells. PA and RES have synergetic effect in the inhibition of gastric carcinoma cells. Compared with PA monotherapy, the combination of RES and PA can reduce the dosage of PA.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2008年第9期815-817,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
关键词
胃癌
紫杉醇
白藜芦醇
Gastric cancer
Paclitaxel
Resveratrol
