摘要
目的检测一种植物类雌激素杨梅素对人结肠癌细胞系RKO生长的影响,并初步探讨其作用机制。方法采用噻唑蓝(MTT)比色法检测杨梅素对RKO细胞系生长的影响;流式细胞仪技术检测杨梅素对细胞周期的影响;半定量反转录聚合酶链反应(RT-PCR)确定杨梅素对抑癌基因p16INK4a表达的影响。结果MTT法显示杨梅素处理组对结肠癌细胞系生长有明显抑制作用;流式细胞仪分析结果显示,经杨梅素处理后RKO细胞周期明显阻滞在G0/G1期;RT-PCR结果显示杨梅素恢复了RKO细胞系p16INK4a的转录。结论杨梅素对RKO细胞系生长有明显的抑制作用,杨梅素可能通过上调抑癌基因p16INK4a的表达使RKO细胞周期阻滞于G0/G1期。
Objectives： To study the effect of myricetin,a phytoestrogen,on the proliferation of human colorectal cancer cell line RKO and its regulation on the expression of tumor suppressor gene p16INK4a.Methods： RKO cells were treated with different concentrations of myricetin.The cell viability was detected with MTT assay and the mRNA level was determined by semi-quantitative RT-PCR.Results： Myricetin inhibited the growth of RKO cells and resulted in cell-cycle arrest in the G0/G1 phase.Myricetin reversed the expression of tumor suppressor gene p16INK4a. Conclusion： The results suggest that myricetin may inhibit cell proliferation by restoring the expression of tumor suppressor gene p16INK4a and blocking cell cycle progression.
出处
《生殖医学杂志》
CAS
2008年第4期290-293,共4页
Journal of Reproductive Medicine
基金
国家重点基础研究发展计划(2007CB511905)资助
