摘要
目的了解广州市一起水禽H5N1疫情中高致病性禽流感H5N1亚型病毒血凝素(HA)基因的特点以及与其他毒株序列间的关系。方法自疫点采集97份咽拭子标本及84份环境拭子标本,实时荧光RT-PCR检测H5核酸,从阳性标本中扩增全长HA基因并作序列测定,同2006年广州市及国内其他地区报道的人禽流感病毒HA基因进行比较并作系统发生分析。结果从一份环境拭子中扩增获得一株H5N1禽流感病毒HA基因,系统发生树分析发现与广州市2006年人禽流感病毒HA序列一致性较高,且与国内其他地区报道的H5N1病毒HA基因属于同一组;从基因序列推导出HA的氨基酸序列,发现其受体特异性为禽源的,蛋白酶水解位点含有多个连续的碱性氨基酸,符合高致病性禽流感病毒的特征。结论引发此次禽间禽流感的为高致病性H5N1病毒,和广州市人禽流感的H5N1病毒在遗传上有紧密的亲缘关系。禽源性的H5N1病毒仍存在感染人类的可能性,禽类从业人员目前处于感染高致病性禽流感的高危状态,进一步加强对活禽市场和禽类从业人员的监测,对于预防禽流感的流行具有重要意义。
[Abstract] Objective To characterize the hemagglutinin （HA） gene of high pathogenic avian influenza viruses （HPAIV） from an outbreak in water fowl in Guangzhou, and to analyse the phylogenetie relation with other H5N1 viruses isolated previously. Method 97 throat swabs and 84 environment swabs were collected from the outbreak site. H5 was detected by real-time fluorescent RT-PCR. Whole lenth HA gene was amplified from the positive specimen, thereby sequenced and phylogenetic analyzed with sequences related to human HPAIV infection in Glmngzhou in 2006. Result One HA gene was amplified from a environment specimen. The phylogenetic analysis showed the sequence was genetically close to two sequences gained from human HPAIV infection in Guangzhou in 2006, and these three HA sequences belonged to same clade with other HA sequences from China. The translated amino acid sequence showed the receptor specificity was still avian region. A multiple basic amino acid residuals motif was found in connecting peptide between HA1 and HA2, which was consistent with the feature of high pathogenicity. Conclusion The virus from this outbreak in water fowl was high pathogenic and avian region, butgenetically close to human-infected HSNI viruses. It implicated that the probability of infection of human is still remained, and the avian occupational population is under a huge risk. The surveillance on the live bird markets and avian occupational population is very important for predicting and prevention of the epidemic of avian influenza.
出处
《热带医学杂志》
CAS
2008年第8期765-768,共4页
Journal of Tropical Medicine
基金
国家自然科学基金(No.30600724)
广东省自然科学基金研究团队项目(No.06201946)
广州市科技计划项目(No.2006J1-C0141)
广州市医学科学技术研究重点项目(No.2006-ZDi-10)
