摘要
背景:目前,尚未在体外环境中构建出与正常气管软骨生物学和机械特性相似的组织工程化气管软骨。目的:测定体外和体内培养环境中软骨细胞-DegraPol支架复合物的机械力学值,探讨提高管状组织工程软骨力学适应性的培养方法。设计、时间及地点:单一样本观察,于2006-12/2008-02在深圳市人民医院医学研究中心完成。材料:分离培养2周龄SD幼鼠的剑突软骨细胞及肌成纤维细胞,分别接种DegraPol管状支架上,制备软骨细胞-支架复合物及肌成纤维细胞-支架复合物,以空白支架为对照。方法:将3组支架在2种培养条件下培养:①体外静态培养3周和6周。②体外培养3d后置于同系大鼠体内大网膜包裹1,2和6周。主要观察指标:体外/体内培养条件下的软骨细胞-支架复合物的机械力学强度。结果:体外培养条件下,最大应力和应变值培养6周者低于培养3周(P<0.05),软骨细胞-支架组的支架最大应力值和应变值高于其他2组(P<0.05)。体内培养条件下,3组最大应力值随培养时间延长而增加,培养6周的数值均高于培养1周,软骨细胞-支架组最大应力值高于其他2组(P<0.05);3组最大应变值随培养时间延长而降低,培养6周的数值均低于培养1周(P<0.05)。无论体内还是体外培养获得的组织工程软骨的生物力学强度均远远低于正常大鼠气管软骨。结论:体内培养方式有利于提高工程化组织的力学强度,但单纯静态体外培养或者体内培养获得的软骨细胞-支架复合物力学强度尚不能用于原位气管移植。
BACKGROUND： Currently, we haven＇t cultivated a tissue-engineered tracheal cartilage in vitro similar to normal tracheal cartilage in biological and mechanical characteristics. OBJECTIVE： To determine the mechanical mechanics of the chondrocytes-DegraPol scaffold cultivated in vitro and in vivo, to investigate the culturing condition of improving the mechanical adaptability of tube tissue engineering tracheal cartilage. DESIGN, TIME AND SETTING： A single sample observation was carried out in the Medical Science Research Center of Shenzhen People＇s Hospital （Shenzhen, Guangdong, China） from December 2006 to February 2008. MATERIALS： SD rats aged 2 weeks were used to isolate and culture the xiphoid chondrocytes and myofibroblasts, which were then seeded on DegraPol scaffolds to prepare the chondrocyte-scaffold composite and myofibroblast-scaffold composite, respectively. While the blank scaffolds served as control group. METHODS： Three kinds of scaffolds were cultivated in vitro for 3 and 6 weeks, and cultivated in vivo for 1, 2 and 6 weeks following 3-day static culture in vitro, respectively. MAIN OUTCOME MEASURES： The mechanical mechanics analysis of chondrocyte-scaffold composites cultured in vitro and in vivo were detected. RESULTS： The maximal stress and strain of three groups cultivated 6 weeks were lower than those cultivated 3 weeks （P 〈 0.05）, chondrocyte-scaffold composites showed higher values compared to myofibroblast-scaffold composite and blank scaffold cultured in vitro （P 〈 0.05）. Cultured in vivo, the maximal stress of three groups increased with the time going, which was higher at 6 weeks than at 1 week. Chondrocyte-scaffold composites still showed a higher value than myofibroblast-scaffold composite and blank scaffold （P 〈 0.05）. The maximal strains of three groups decreased with the time going, which were lower at 6 weeks than at 1 week （P 〈 0.05）. The biomechanical strength of tissue-engineered cartilage cultured in vivo and in vitro was remarkably lower than the normal rat tracheal cartilage. CONCLUSION： The mechanical characteristics of tissue engineering benefits from the culture in vivo. However, the biomechanical strength of chondrocyte-scaffold cultivated by static condition in vitro or in vivo is too weak to be applied on tracheal transplantation in situ.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第27期5291-5294,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金资助项目(30500499)~~
