摘要
目的检测胃溃疡患者胃泌素(gastrin,GAs)mRNA的表达,探讨胃泌素在胃溃疡发病中的作用。方法取手术切除的胃溃疡新鲜标本15例,分别在胃溃疡边缘及切端(相对正常胃组织)取材。胃镜胃溃疡标本15例。运用逆转录PCR(RT- PCR)的方法检测2个不同部位中GAS mRNA的表达,其光密度值(OD)与内参照β-actin的光密度值比较,分析2个部位的OD比值的不同。结果胃溃疡标本的2个不同部位均有GAS mRNA的表达,在胃溃疡边缘GAS mRNA的OD比值明显高于胃溃疡切端相对正常胃组织的OD值,差异有显著性(P<0.05)。结论在基因水平证实了GAS在胃溃疡中的表达,而且表明GAS等胃肠激素在胃溃疡的发病过程中起着非常重要的作用。
Objective To investigate the expressions of gastrin mRNA in the gastric ulcer, and to explore its role in the pathogenesis of the gastric ulcer. Methods The 30 gastric ulcer tissues and the 20 normal gastric tissues were studied by using the reverse transcriptase-polymerase chain reaction （ RT-PCR ）. To compare the differences of the expressions of gastrin mRNAs. Results There were the expressions of gastrin mRNA both in the gastric ulcer and the normal tissue. The OD ratio of gastrin mRNAs in the margins of gastric ulcer were higher than in the normal gastric tissues （ P 〈0.05 ）. Conclusions GAS play important role in the pathogenesis of the gastric ulcer.
出处
《潍坊医学院学报》
2007年第6期517-518,F0003,共3页
Acta Academiae Medicinae Weifang
