摘要
目的：了解烧冲复合伤早期肺组织细胞间粘附分子－１（ＩＣＡＭ－１）的表达变化规律及其与多形核白细胞（ＰＭＮ）在肺内聚集的关系．方法：测定肺组织髓过氧化物酶（ＭＰＯ）以反映ＰＭＮ肺内聚集的情况；用地高辛标记ＩＣＡＭ－１ｃＤＮＡ探针进行肺组织原位杂交；用ＳＰ法进行肺组织ＩＣＡＭ－１免疫组化染色．结果：各致伤组动物伤后均有不同程度的肺组织ＭＰＯ活性增高，其中以复合伤组最明显，肺组织ＩＣＡＭ－１ｍＲＮＡ及其蛋白质表达均较对照组显著增多，以伤后６小时最为明显．结论：烧冲复合伤早期肺ＩＣＡＭ－１增多可能与ＰＭＮ的肺内聚集有关．
Aim: To realize changes of pulmonary intercellular adhesion molecule 1(ICAM 1) expression and accumulation of pulmonary polymorphonuclear neutrophil (PMN) in the early stage of burn blast combined injury. Methods : Pulmonary myeloperoxidase activity was assayed to show accumulation of PMN in the lung. Lung tissue in situ hybridization was determined by digoxin labeled ICAM 1 cDNA probe. Immunohistochemistry of ICAM 1 in the lung was measured. Results: Pulmonary myeloperoxidase activity of each injured group increased after injury. The most obvious changes was showed in burn blast combined injury. The expression of ICAM 1 mRNA and its protein of the lung in each injured group were markedly increased than that of the control. The peak level was observed at 6 hours after injury. Conclusion: The process of accumulation of PMN in the lung might be related to the increase of ICAM 1 expressing in the lung at the early stage of burn blast combined injury.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
1997年第5期282-284,共3页
Chinese Journal of Trauma
关键词
烧伤
冲击伤
复合伤
细胞间粘附分子
PMN
Intercellular adhesion molecule 1 Lung Polymorphonuclear neutrophil Burn blast combined injury
