摘要
用ＲＰ－ＨＰＬＣ测定人血浆中洛伐他汀浓度。以甲醇－水（８３∶１７，Ｖ／Ｖ）为流动相，色谱柱采用ＨＹＰＥＲＳＩＬＢＤＳ－Ｃ１８（５μｍ）不锈钢柱，紫外２３７ｎｍ波长检测。血浆样品经环己烷－异丙醇（９５∶５）萃取浓缩后进样测定。洛伐他汀浓度在２．５～８０ｎｇ／ｍｌ范围内线性良好，ｒ＝０．９９６８。测定含洛伐他汀２０．０ｎｇ／ｍｌ的血浆样品，其日内（ｎ＝７）及日间（ｎ＝７）的ＲＳＤ分别为９．８％和８．５％。回收率平均为（１０１．３±５．５）％。测定了１０名健康志愿者单次ｐｏ洛伐他汀片剂８０ｍｇ后不同时间的血药浓度，计算了相应的药动学参数。
A simple and rapid RP HPLC method has been developed for determining lovastatin in human plasma. The analytical column was packed with HYPERSIL BDS. The mobile phase was methanol water(83∶17, V/V ). The lovastatin in plasma sample was extracted by cyclohexane isopropyl alcohol(95∶5). The compounds were quantitated using a ultraviolet detector operated at 237 nm which allowed determination of 2.5￣80 ng/ml of lovastatin with reproducibility. The limit of detection was 1 ng/ml. Intra day and inter day RSD for assaying the plasma sample containing 20 ng/ml concentration of lovastatin were 9.8%( n =7) and 8.5%( n = 7), respectively. The mean recovery was 101. 7%. The pharmacokinetics of lovastatin in ten healthy volunteers after oral administration of 80 mg was studied.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1997年第5期288-290,共3页
Journal of China Pharmaceutical University
