摘要
应用原位杂交（ISH）方法检测了12例丙型肝炎病毒（HCV）感染者肝组织中HCVRNA，以研究丙肝病毒的定位及其与肝细胞病变的关系。结果：12例患者中9例（75％）阳性，阳性信号主要位于肝细胞浆中；切片经RNA酶消化后进行杂交，阳性信号消失但DNA酶预处理对结果无明显影响；阳性细胞可位于炎症坏死灶附近，但也可与炎症反应无明显关系。结果表明原位杂交方法对研究HCV生物学特性及致病机制有一定价值。
To study the location of hepatitis C virus (HCV) in liver and its relationship with the inflammation of liver tissue, we detected HCV RNA in liver tissue of a group of patients with HCV infection by in situ hybridization (ISH). Results: Among the 12 patients, 9 (75%) were positive for HCV RNA; The staining signal was located mainly in the cytoplasm of liver cells ; The specificity of the result was demonstrated by pretreating the positive sectipns with RNase and DNase, respectively. As expected, the positive signal was disappeared after RNase treatment, but not affected by DNase treatment. The positive cells can be distributed in or near the necroinflammatory area, but most f them had no apparent topographical relationship with necroinflammation. The results suggest that ISH is a useful method in the study of physiological characteristics and pathogenesis of HCV.
出处
《同济医科大学学报》
CSCD
1997年第5期365-367,共3页
Acta Universitatis Medicinae Tongji
基金
国家八.五攻关资助!No.85-916-01-05-6
