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应用化学发光自显影技术检测甲型肝炎病毒和人IgM 被引量:6

Application of Chemiluminescent Photographic Detection in Identification of HAV and Human IgM
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摘要 运用化学发光自显影技术和化学发光酶免疫分析法,可检出15.60pg辣根过氧化物酶;在甲型肝炎病毒和人IgM的免疫测定中,检出限分别为1:128(滴度)和6.4×104(人标准血清稀释倍数),较酶连接免疫吸附测定比色法灵敏4~8倍。 Using chemiluminescent photographic detection and chemiluminescent enzyme immunoassay,we detected 15.60pg HRP;and in immunoassays for HAV and human IgM, its detection limit was 1:128 (titer) and 6.4×104 (dilution of normal human serum) respectively, which was 4~8 times more sensitive than ELISA with colorimetry
出处 《同济医科大学学报》 CSCD 1997年第5期362-364,370,共4页 Acta Universitatis Medicinae Tongji
基金 国家自然科学基金!No.39470214
关键词 甲型肝炎病毒 免疫球蛋白M CPD ELISA chemiluminescence enzyme-linked immunosorbent assay horseradish peroxidase imaging technics
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参考文献1

  • 1杨秀岑,中华医学检验杂志,1993年,16卷,1期,8页

同被引文献14

  • 1李冬田,李秋香,郑玉廷,佟惠春,任中原.直接酶标HBVDNA探针及化学发光自显影检测技术的应用[J].中华微生物学和免疫学杂志,1994,14(6):430-432. 被引量:3
  • 2Zhai J H,Biotechnol Adv,1997年,15卷,1期,43页
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  • 7Matson R S,Rampal J,Pentoney S L et al.Biopolymersynthesis of polypropylene supports: oligonucleotide array. Analytical Biochemistry . 1995
  • 8Zammatteo N,Girardeaux C,Delforge D et al.Amination of polystyrene microwells: application to the covalent grafting of DNA probes for hybridization assays. Analytical Biochemistry . 1996
  • 9方华丰,周宜开.生物素—亲和素系统在生物传感器中的应用[J].化学传感器,1997,17(3):173-183. 被引量:5
  • 10梁雁,林汉华,王慕逖,袁津伟.化学发光法测定辣根过氧化物酶浓度的实验条件探讨[J].同济医科大学学报,1998,27(6):445-447. 被引量:5

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