摘要
探讨了超声波辅助提取盾叶薯蓣皂苷元的优化工艺,主要考察了超声波作用对薯蓣皂苷元得率的影响,并与预发酵法结果进行比较。通过单因素及正交试验考察了不同处理条件对薯蓣皂苷元得率的影响,筛选出超声波辅助提取盾叶薯蓣皂苷元的最优工艺条件为:10g盾叶薯蓣粗粉,固液比1∶10(g∶mL,下同),萃取剂乙醇的体积分数为70%,超声波功率100W,超声波提取2次,每次30min,在此条件下,所得皂苷元得率为2.57%。而传统方法经预发酵24h后,皂苷元得率仅为2.13%。与预发酵法相比,超声波辅助法可以大大缩短提取时间,而且薯蓣皂苷元得率也有比较明显的提高。
Ultrasound-assisted extraction of diosgenin from rhizome of Dioscorea zingiberensis C. H. Wright was optimized. Compared with the traditional spontaneous fermentation, the effects of operating conditions were studied according to single factor and orthogonal design. The optimal condition was achieved as follows：crude powder of D. zingiberensis 10 g materials to solvent ratio 1 ： 10 （g： mL）, 70 % alcohol, extracted twice under ultrasonic power of 100 W, each time for 30 min. Under the above conditions, diosgenin can be obtained at the yield of 2.57 %. By contrast, the maximum yield of diogenin is only 2.13 % by traditional method after spontaneous fermentation for 24 h. Therefore, ultrasonic-assisted technology was more effective with the advantages of much shorter extracting time and higher yield of diosgenin.
出处
《林产化学与工业》
EI
CAS
CSCD
2008年第4期103-107,共5页
Chemistry and Industry of Forest Products
基金
天津市应用基础及前沿技术研究计划重点项目(08JCZDJC15300)
关键词
盾叶薯蓣
皂苷元
超声波辅助提取
Dioscorea zingiberensis C. H. Wright
diosgenin
ultrasound-assisted extraction
