摘要
目的:研究猪急性心肌梗死(AMI)后囊性纤维化跨膜转运调节体(CFTR)氯通道基因表达的变化,探讨急性心肌梗死后早期室性心律失常发生的分子机制。方法:通过结扎猪左前降支远端1/3～1/2处2h然后再灌注建立AMI模型(AMI组),同时设立相应的假手术组。术后24h取左心室梗死区、边缘区和正常区内层、中层和外层心肌(假手术组取对应位置心肌),应用逆转录-聚合酶链反应(RT-PCR)半定量分析CFTR氯通道基因表达的改变。结果:与假手术组相比,AMI组CFTRmRNA表达在梗死区三层心肌中均明显下降(P<0.05),在边缘区三层心肌中均明显上升(P<0.05),在正常区三层心肌中均没有显著性改变(P>0.05)。AMI组CFTRmRNA表达在梗死区和边缘区的三层心肌之间以及在梗死区、边缘区和正常区同一层心肌之间差异有显著性(P<0.05)。结论:AMI后CFTR氯通道基因表达在梗死区、边缘区和正常区心肌之间以及局部心肌三层间呈不均一性改变,可能是AMI后室性心律失常发生的分子生物学基础之一。
Objective To investigate the changes of the expression of cystic fibrosis transmembrane conductance regulator （CFTR） gene induced by acute myocardial infarction （AMI） in pigs. Methods The pig model of A MI was established by ligating left anterior descending coronary artery for 2 h followed with reperfusion. 24 h after surgery, semiquantitative reverse transcriptase-polymerase chain reaction （RT-PCR） was used to measure the CFTR mRNA in each group. Results Compared with the sham-group, CFTR mRNA expression was significantly down-regulated in all three layers of infract zone（P 〈 0.05） ,but significantly up-regulated in all three layers of border zone（P 〈 0.05） of AMI group, and no significant change of CFTR mRNA expression was detected among three myocardium layers of normal zone （P 〉 0.05）. In AMI group, the CFTR mRNA expression was significantly different among three myocardium layers of infarct zone or border zone, and in different zones of the same myocardium layer （P 〈 0.05）. Conclusion The heterogeneous expression of CFTR gene in different zones and myocardium layers may be one of the molecular mechanisms involved in ventricular arrhvthmias induced by AMI.
出处
《实用医学杂志》
CAS
2008年第16期2751-2753,共3页
The Journal of Practical Medicine
基金
湖北省"十一五"重大科技攻关项目(编号:2006AA301A04)
关键词
心肌梗塞
心律失常
心血管病学
氯通道
囊性纤维化跨膜转运调节体
Myocardial infarction Arrhythmia Cardiology Chloride channels Cystic fibrosis transmembraneconductance regulator
