摘要
以我国山东无花果树(Brunswike,原产法国Ficus carica)树叶为原料,采用缓冲液抽提、硫酸铵分级、DEAE-Sepharose Fast Flow阴离子交换层析和Sephacryl S-100HR分子筛层析分离纯化得到电泳纯CuZn-SOD,并对其酶学性质进行研究.
A CuZn-SOD from Ficus carica leaves was separated and purified with ammonium sulfate fractionation, DEAE-Sepharose fast flow chromatography and Sephacryl S-100 HR chromatography techniques. One major bright band was achieved on polyacrylamide gel electrophoresis stained with nitroblue tetrazolium （NBT）. The molecular weight of the enzyme subunit was determined by SDS-PAGE to be about 17800. We investigated the physical and chemical properties of the enzyme, and characterized its kinetic properties, such as the optimal pH, optimal temperature and the effect of chemical reagents andmetal ions on the SOD. The results show that the stability of this purified SOD appeared to be better than other SODs reported. The analysis results of circular dichroism spectrum reveals that the enzyme possessed approximate 12.78% of α-helix in its secondary structure.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2008年第8期1588-1591,共4页
Chemical Journal of Chinese Universities
关键词
无花果树树叶
超氧化物歧化酶
分离纯化
Ficus carica leaves
Superoxide dismutase
Separation and purification
