摘要
利用电喷雾傅里叶变换离子回旋共振质谱研究一系列双β-咔啉化合物与DNA的非共价结合。发现化合物1—5与5种不同序列的12-mer双链DNA均有明显的非共价结合，并且有两个β-咔啉环之间连接碳链的长度对此类化合物与双链DNA的非共价结合活性有明显影响．同时对此类化合物对于DNA非共价结合的序列选择性进行了讨论．另外还利用电喷雾离子阱质谱研究了双β-咔啉化合物2和4与4种单核苷酸的非共价结合．
The non-covalent complexes of five bis-β-carbolines alkaloids with five different double-stranded oligodeoxynucleotides were investigated via electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry. These five antitumor compounds all showed DNA binding abilities. The binding affinities in the order of 2 〉 3,4 〉 5,1 were obtained, which mean that the length of the linkage chain between two β- carbolines has a remarkable effect on the formation of the non-covalent complexes. The competition binding experiments results were almost the same as that determined by relative ion abundances. The structure-activity relationships and sequence selectivity were discussed. The non-covalent bindings between the bis-β-carbolines 2, 4 and the nucleotide were then investigated by ESI-MS.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2008年第8期1578-1582,共5页
Chemical Journal of Chinese Universities
基金
上海市卫生局青年科研基金(批准号:2006Y14)资助
关键词
双β-咔啉
DNA
核苷酸
非共价结合
电喷雾傅里叶变换离子回旋共振质谱
Bis-β-carbolines
DNA
Nucleotide
Non-covalent interaction
Electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry
