摘要
目的调查外科手术切口术后感染的病原菌及其耐药性。方法采集2001年1月-2006年12月从外科手术后伤口感染分泌物分离的病原菌,用全自动微生物分析仪进行菌种鉴定和药物敏感试验。结果6年间从手术切口感染标本中共培养出246株病原菌,革兰阴性杆菌142株,占57.7%;革兰阳性球菌84株,占34.1%;真菌20株,占8.2%;前5位的细菌依次为大肠埃希菌、金黄色葡萄球菌、铜绿假单胞菌、肺炎克雷伯菌和鲍氏不动杆菌;耐甲氧西林金黄色葡萄球菌(MRSA)和表皮葡萄球菌分离率为74.5%和83.3%;产超广谱β-内酰胺酶(ESBLs)大肠埃希菌和肺炎克雷伯菌分离率为45.1%和35.0%;耐亚胺培南铜绿假单胞菌和鲍氏不动杆菌的分离率为34.6%和38.1%,经药物敏感试验测定发现以上耐药菌株均显示了严重的多药耐药性;但万古霉素和亚胺培南仍具有良好的抗菌作用。结论外科切口术后感染较为严重,感染菌株多为多药耐药菌株。
OBJECTIVE To investigate the resistance status of pathogens from surgical incision infection. METHODS The secretion of infected wounds was cultured to detect pathogens by routine methods from Jan 2001 to Dec 2006. The identifications and antimicrobial-susceptible tests of pathogens causing incision infection were determined by Full Automated Analyzer. All data were analyzed retrospectively. RESULTS A total of 246 pathogen strains were cultured from the secretion of surgical incision infection in 6 years, of which Gram-negative bacilli, Gram-positive cocci and fungi accounted for 57.7%, 34. 1% and 8.2%, respectively. The first place of isolates was Escherichia coli , followed by Staphylococcus aureus ,Pseudornonas aeruginosa , Klebsiella pneumoni-ae,Acinetobacter baurnannii, etc. 74. 5% of S. aureus isolates and 83. 3% of S. epiderrnidis isolates were resistant to oxacillin. 45.1% of E. coil of the isolates and 35.0% of isolates of K. pneumoniae were extended-spectrum β-lactamases producing. Almost all of the detected resistant strains showed serious multiple resistance. Vancomyein and imipenem still had better activity for antimicrobial multiple resistant bacteria. CONCLUSIONS Surgical incision infection is serious. Infection surveillance should be taken to control surgical infection. Pathogens infected surgical incision are multi-resistant to antibiotics.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2008年第8期1186-1188,共3页
Chinese Journal of Nosocomiology
关键词
手术切口
感染
病原菌
耐药率
Surgical incision
Infection
Pathogens
Antimicrobial resistance
