摘要
本文研究了各种影响酶促反应速度的因素对从猪、牛肾中分离得到的氨基酰化酶拆分ＤＬ－Ａｌａ制备Ｄ－Ａｌａ的影响，结果表明，酶促反应的最适条件为：Ｎ－乙酰－ＤＬ－Ａｌａ的浓度为０．２ｍｏｌ／Ｌ，酶用量７００ｕ／ｍｍｏｌ底物，反应温度３７℃，ｐＨ７．０，反应时间５ｈ。牛肾氨基酰化酶和猪肾氨基酰化酶的拆分率和Ｄ－Ａｌａ得率最高分别为９０％和８４％；８９．７％和６６．１％，表明牛肾氨基酰化酶的拆分率和得率均优于猪肾氨基酰化酶。
Aminoacylases (EC 3.5.1.14) were extracted from pig and cattle kidney and were used to resolute N-Acyl-Ala to obtain pure DAla. A number of factors have effects on the resolution reaction catalysed by two kinds of aminoacylases.The optium resolution condition was observed by using these aminoacylases 1.5 mg/mmol substract.The optium pH,reaction temperature, reaction time and the concentration of substract were 7.0,37℃,9 hours and 0.2 mol/L. The result also showed that the resolution rate and DAla recovery catalysed by cattle kidney aminoacylase were higher than those by pig kidney aminoacylase.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1997年第6期358-361,共4页
Journal of China Pharmaceutical University
