摘要
目的研究平滑肌收缩调控蛋白－调宁蛋白在胆结石动物Ｏｄｄｉ括约肌中的表达，从分子水平上阐明胆结石形成的机制。方法饲予豚鼠高胆固醇饮食，分别在３０天和６０天取出Ｏｄｄｉ括约肌，应用逆转录聚合酶链反应（ＲＴ－ＰＣＲ）和蛋白质免疫印迹法（Ｗｅｓｔｅｒｎｂｌｏｔｔｉｎｇ）检测动物Ｏｄｄｉ括约肌调宁蛋白基因的表达水平。结果发现在结石形成过程中，调宁蛋白基因的表达水平逐渐下降，３０天组和６０天组的调宁蛋白含量和ｍＲＮＡ水平均比对照组低，且６０天组比３０天组低，而另一调控蛋白肌球蛋白的表达水平无明显变化。结论调宁蛋白表达水平的下降，可能使Ｏｄｄｉ括约肌的张力增加，加重胆汁淤滞，促使胆结石的形成。
bjectives To study on the expression of calponin in an animal model of gallstone disease and investigate the molecular mechanisms of gallstone formation. Methods After feeding a highcholesterol diet to guinea pigs, Oddi′s sphincters were disseced on day 30 and day 60 respectively. We used RTPCR, westernblotting to evaluate expression level of calponin gene. Results Downregulation of calponin gene expression was observed in animals with gallstone. The levels of both protein and mRNA expression for calponin on day 30 and day 60 were lower than those of control group with the level from day 60 lower than that from day 60, while myosin expressions were relatively stable. Conclusion Our results indicated that the decrease of calponin could increase the pressure of sphincter of Oddi, aggravate the stasis of bile and promote the gallstone formation.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1997年第11期812-814,共3页
National Medical Journal of China
基金
国家自然科学基金
国家教育委员会基金
