摘要
目的:探讨何首乌二苯乙烯苷(TSG)调节胆固醇代谢作用机制。方法:体外培养Bel-7402细胞株,待细胞长满80%后无血清培养基饥饿细胞24h,分别加入1、10、100μM的二苯乙烯苷及10μM的阿托伐他汀,RT-PCR检测给药24h后细胞内低密度脂蛋白受体(LDLR)、β-羟β-甲基戊二酰辅酶A还原酶(HMGCR)、胆固醇7α-羟化酶(CYP7A1)、酰基辅酶A胆固醇酰基转移酶(ACAT)的基因表达。结果:和空白对照组相比,二苯乙烯苷组能明显升高肝细胞表面LDLR的表达(P<0.05,P<0.01),二苯乙烯苷中、高剂量组与阳性药组相比无明显差异(P>0.05);二苯乙烯苷组与阳性药组均增加细胞HMGCOAR表达,各组之间无明显差异;阿托伐他汀组降低ACAT的表达(P<0.05),二苯乙烯苷组则无明显作用;阿托伐他汀组Cyp7A1表达高于二苯乙烯苷组,与对照组相比无显著性差异。结论:二苯乙烯苷可能是通过抑制细胞内胆固醇的合成及升高低密度脂蛋白受体的表达而起到降血脂作用。
Objective：To research the mechanism of Stilbene Glucoside from Polygonum multiflovum Thumb on hyper-lipemia. Methods：Bel-7402 cells were grown in 1640 medium with 10% FBS for 24hrs. The medium was replaced with serum-free medium and cells were incubated for another 24 hrs. 1 umol/L,10 umol/L,100 umol/L tsg and 10 umol/L atorvastatin were added in medium. Gene expression of LDLR, HMGCOAR, CYP7A1 and ACAT were measured. Results ： compared with the control group,The expression of LDLR, HMGCOAR were increased significantly by each dose of TSG （ P 〈 O. 05,P 〈 0. 01 ）. ACAT mRNA expression were reduced by Atorvastatin （P 〈 0.05 ）, TSG did not have effect on the expression of ACATcell（P 〉0. 05） , both TSG and Atorvastatin did not have effect on the expression of Cyp7al. Conclusions ： TSG inhibite cholesterol synthesis in cell, elevate the expression of low - density lipoprotein receptor, and this may be the mechanism of bypolipidemic by TSG.
出处
《中华中医药学刊》
CAS
2008年第8期1687-1689,共3页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(902409011)
北京市自然科学基金资助项目(7061002)
