摘要
用胰酶消化的中心浸液作基础培基,添加新生小牛血清、自制新鲜酵母浸液。从NGU患者的中段尿、慢性前列腺炎患者的前列腺液和男性不育患者的精液中分离尿素支原体共447份。其中NGU119例,68例为阳性(34.176％);慢性前列腺炎159例,33例为阳性(20.7％);男性不育患者89例,54例为阳性(60.6％)。用扫描电镜观察菌体呈卵圆形或球形,直径0.3～0.5μm,不呈长丝状或链状。在液体培基中,能分解尿素,产生NH_3,培基由黄色变为粉红色。在固体培基中,低倍观察菌落呈“油煎蛋”状。适宜的PH为6.0～6.5。适宜的气体为10％CO_2和90％N_2,我们认为尿素支原体的鉴定应分为两步;首先在液体培基中观察颜色变化;然后在固体培基上观察典型菌落形态。
A medium was prepared using pancreatic digest of beef heart as the base, supplemented with new-born calf serum and self-made fresh yeast extract. The Ureaplasma urealyticum were isolated from the 447 patients with midstream of nongonococcal urethritis: prostatitic massage of chronic prostatitis and semen of male infertility, among 199 cases of nongonococcal urethritis, 68 cases were positive (34.176%), 159 cases of chronic prostatitis, 33 cases were positive (20.7%), 89 cases of male infertility, 54 cases were positive (60.6%). The Ureaplasma collected from liquid medium observed by SEM appeared to be ovoid or spherical, with a diameter of 300-500nm, and no filamentous form or chain was observed. In liquid media containning 0.002% phenol red 0.1%urea, urea was decomposed and NH_3 liberated, changing the color of the media from yellow to pink. Colong on solid media, observed by 10x objective presented a typical 'fried agg' appearance. The optimal PH is 6.0-6.5. The organisms require a gaseous requirement of 10% CO_2 and 90% N_2 for their growth. The authors recommend a 'two step' procedure for the identification of U urealyticum first color change in the acid liquid media and then confirmed by typical colonical morphology on solid media.
关键词
尿素支原体
生殖泌尿道
感染
分离
ureaplasma urealyticum
isolation and culture
genito-urinary tract infection
