摘要
[目的]观察2型糖尿病患者结膜上皮细胞中转化生长因子-β1(TGF-β1)和核转录因子-κB(NF-κB)的表达情况.[方法]选择2型糖尿病患者92例和正常人70例,任意选取一眼进行泪膜破裂时间(BUT)、基础泪液分泌试验及角膜荧光染色试验,并应用免疫组织化学方法检测结膜上皮细胞中TGF-β1及NF-κB的表达.[结果]糖尿病组患者BUT值小于10 s和基础泪液分泌值小于5 mm者所占比例较对照组明显升高,角膜荧光染色程度较对照组明显增强.结膜上皮细胞TGF-β1和NF-κB的染色程度均较对照组明显增强;糖尿病组患者结膜上皮细胞TGF-β1与NF-κB的染色程度呈正相关.[结论]2型糖尿病患者BUT值和基础泪液分泌试验值降低,角膜荧光染色程度增强,是干眼症易患人群.
OBJECTIVETo study the expression of transforming growth factor-β1（TGF-β1） and nuclear factor-κB（NF-κB） on conjunctiva endepidermis cells in patients with diabetes mellitus（DM）.METHODS92 patients of DM and 70 cases of health adult were randomly selected.One eye in patients was selected to do the tear film break-up time（BUT）,Schirmer test and cornea fluorescein staining,and the expression of NF-κB and TGF-β1 on bulbar conjunctiva endepidermis was detected by immunohistochemistry.RESULTSThe ratios of BUT value（〈10s） and Schirmer value（〈5mm） in DM increased significantly as compared with the control group,and the degree of cornea fluorescein staining in DM was higher than that in control group.The staining level of NF-κB and TGF-β1 of bulbar conjunctiva endepidermis in DM was higher than that in control group and was positive correlation.CONCLUSIONIn type 2 DM,the value of BUT and Schirmer decrease and the degree of cornea fluorescein staining increase is incident to the xeroma.
出处
《延边大学医学学报》
CAS
2008年第2期105-107,共3页
Journal of Medical Science Yanbian University
关键词
糖尿病
2型
转化生长因子Β
干眼症
diabetes mellitus,type 2
transforming growth factor beta
xerophthalmia
