摘要
目的探讨辣椒素联合5-Fu对食管鳞癌Eca109和EC9706细胞增殖、耐药的影响。方法将EC9706和Eca109细胞分为对照组、辣椒素组、5-Fu组和联合用药组,MTT法检测72 h后细胞的增殖情况及辣椒素联合应用不同浓度5-Fu对食管鳞癌细胞增殖的影响。结果MTT实验表明,辣椒素与5-Fu联合应用可明显抑制Eca109和EC9706细胞的增殖,与单独使用辣椒素或5-Fu组相比,细胞存活率显著降低;对照组和辣椒素组细胞的存活率均随着5-Fu浓度的增加而下降,但在同一浓度,辣椒素与5-Fu联合应用可明显提高食管鳞癌细胞对化疗药的敏感性。结论辣椒素与5-Fu联合应用,可以增强食管鳞癌细胞对化疗药的敏感性,因此辣椒素有望成为食管癌治疗中的辅助用药。
Objective To investigate ceil proliferation of esophageal squamous cell carcinoma cell lines with capsaicin in combination with 5- Fu. Methods The ESCC cell lines were divided into control group, capsaicin group, 5-Fu group and capsaicin in combination with 5-Fu group. Cell viability was detected with MTT after 72 h. The sensitivity to 5-Fu of the Escc cell lines was evaluated also with MTT. Results The treatment with capsaicin plus 5-Fu, two ESCC cells growth was inhibited drastically compared with capsaicin alone and 5-Fu alone. Proliferations of both cells, control group and capsaicin group, were inhibited in a concentration-dependent manner, while at the same concentration of 5-Fu the viability of capsaicin group cells were significantly suppressed. Conclusion Capsaicin could increases the 5-Fu sensitivity of the ESCC cells, suggesting that eapsaiein might prove useful in the treatment of ESCC.
出处
《肿瘤基础与临床》
2008年第4期280-282,共3页
journal of basic and clinical oncology
