摘要
目的 建立高效液相色谱法测定阿折地平片的含量。方法 采用Diamonsil C18柱（250mm×4．6mm，5um），以磷酸盐缓冲液（磷酸二氢钠0．005mol·L^-1，磷酸氢二钠0．004mol·L^-1）-乙腈（30：70）为流动相，流速1．0mL·min^-1；检测波长256nm；柱温30℃。结果 阿折地平在2．50-40．0ug·mL^-1范围内线性关系良好，r=0．9995，高、中、低3种不同浓度的平均回收率分别为99．8％、100．2％、100．5％，RSD为0．49％、0．57％、0．64％。结论 所用方法简便、准确，可用于阿折地平片剂的质量控制。
OBJECTIVE To establish an HPLC method for determination of content in Azelnidipine Tablets. METHODS The HPLC system consisted of a Diamonsil C18 column（250mm×4. 6mm, 5um）. The mobile phase was phosphate buffer （0. 005 mol · L^-1 disodium dihydrogen phosphate,0, 004 mol · L^-1 disodium hydrogen phosphate）--acetonitrile （30 ： 70）. The flow rate was 1, 0 mL · min^-1. The detection wavelength was 256nm. The column temperature was 30℃. RESULTS The linear range of Azelnidipine was 2. 50-40. 0ug · mL^-1（ r =0. 9995）. The average recovery was 99. 8 %, 100. 2 %, 100. 5 %, RSD was 0. 49%,0. 57%,0.64%. CONCLUSION The method was simple and accurate. It can be used for quality control of content in Azelnidipine Tablets.
出处
《齐鲁药事》
2008年第7期398-399,共2页
qilu pharmaceutical affairs
